Malakhova Anastasia A, Rybin Denis K, Shtertser Alexandr A, Dudina Dina V
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentyev Ave. 10, Novosibirsk 630090, Russia.
Lavrentyev Institute of Hydrodynamics, Siberian Branch of the Russian Academy of Sciences, Lavrentyev Ave. 15, Novosibirsk 630090, Russia.
Micromachines (Basel). 2022 Jul 27;13(8):1187. doi: 10.3390/mi13081187.
The production method of nanoscale detonation carbon (NDC) has recently been developed at Lavrentyev Institute of Hydrodynamics SB RAS. This method uses the reaction of acetylene with oxygen conducted in the detonation mode in fuel-rich acetylene-oxygen mixtures. The morphology and structural features of the NDC particles can be varied by changing the concentration of oxygen in the gaseous mixtures. The particles of NDC can serve as reinforcements in metal matrix composites and additives imparting electrical conductivity to polymer matrix composites. Before NDC can be considered for industrial applications, it is necessary to address the related biological safety concerns. The present work was aimed at determining the cytotoxicity of NDC. The NDC powders with two morphologies (obtained using different acetylene/oxygen ratios) were tested on HEK293A human cells. The NDC powder was added to the culture medium in concentrations ranging from 10 ng/mL to 400 μg/mL. The cell viability was determined by a colorimetric EZ4U test and a real-time cell analyzer xCELLigence. None of the NDC samples showed a cytotoxic effect. The results of this study allow us to recommend NDC as a safe and useful product for the development of advanced carbon-based and composite materials.
俄罗斯科学院西伯利亚分院拉夫连季耶夫流体力学研究所最近开发出了纳米级爆轰碳（NDC）的生产方法。该方法利用乙炔与氧气在富燃料乙炔 - 氧气混合物中以爆轰模式进行反应。通过改变气态混合物中氧气的浓度，可以改变NDC颗粒的形态和结构特征。NDC颗粒可作为金属基复合材料的增强材料以及赋予聚合物基复合材料导电性的添加剂。在将NDC用于工业应用之前，有必要解决相关的生物安全问题。目前的工作旨在确定NDC的细胞毒性。对两种形态的NDC粉末（使用不同的乙炔/氧气比例获得）在HEK293A人细胞上进行了测试。将NDC粉末以10 ng/mL至400 μg/mL的浓度添加到培养基中。通过比色EZ4U测试和实时细胞分析仪xCELLigence测定细胞活力。所有NDC样品均未显示出细胞毒性作用。这项研究的结果使我们能够推荐NDC作为开发先进碳基和复合材料的安全且有用的产品。
