Dechsupa Nathupakorn, Kosintarajit Panida, Kamkan Kanyapak, Khanjina Thanyalak, Sirikul Chonticha, Innuan Phattarawadee, Suwan Authaphinya, Anukul Nampeung, Kantapan Jiraporn
Molecular Imaging and Therapy Research Unit, Faculty of Associated Medical Sciences, Department of Radiologic Technology, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Nanomaterials (Basel). 2022 Aug 13;12(16):2776. doi: 10.3390/nano12162776.
The theranostic agent iron-quercetin complex (IronQ) provides a T1-positive magnetic resonance imaging (MRI) contrast agent. The magnetically IronQ-labeled cells can be used for cell tracking and have active biological applications in promoting cell and tissue regeneration. However, a detailed investigation of IronQ's cytotoxicity and genotoxicity is necessary. Thus, this study aimed to evaluate the possibility of IronQ inducing cytotoxicity and genotoxicity in peripheral blood mononuclear cells (PBMCs). We evaluated the vitality of cells, the production of reactive oxygen species (ROS), the level of antioxidant enzymes, and the stability of the genetic material in PBMCs treated with IronQ. The results show that IronQ had a negligible impact on toxicological parameters such as ROS production and lipid peroxidation, indicating that it is not harmful. IronQ-labeled PMBCs experienced an insignificant depletion of antioxidant enzyme levels at the highest concentration of IronQ. There is no evident genotoxicity in the magnetically IronQ-labeled PBMCs. The results show that IronQ does not potentiate the cytotoxicity and genotoxicity effects of the labeled PMBCs and might be safe for therapeutic and cell tracking purposes. These results could provide a reference guideline for the toxicological analysis of IronQ in in vivo studies.
诊疗试剂铁-槲皮素复合物（IronQ）可作为一种T1阳性磁共振成像（MRI）造影剂。经磁性IronQ标记的细胞可用于细胞追踪，并在促进细胞和组织再生方面具有积极的生物学应用。然而，有必要对IronQ的细胞毒性和遗传毒性进行详细研究。因此，本研究旨在评估IronQ在外周血单个核细胞（PBMCs）中诱导细胞毒性和遗传毒性的可能性。我们评估了用IronQ处理的PBMCs中的细胞活力、活性氧（ROS）的产生、抗氧化酶水平以及遗传物质的稳定性。结果表明，IronQ对ROS产生和脂质过氧化等毒理学参数的影响可忽略不计，表明其无害。在IronQ最高浓度下，经IronQ标记的PMBCs的抗氧化酶水平仅有微不足道的消耗。经磁性IronQ标记的PBMCs中没有明显的遗传毒性。结果表明，IronQ不会增强标记的PMBCs的细胞毒性和遗传毒性作用，可能对治疗和细胞追踪目的是安全的。这些结果可为体内研究中IronQ的毒理学分析提供参考指导。
