Monou Paraskevi Kyriaki, Mamaligka Anastasia Maria, Tzimtzimis Emmanuil K, Tzetzis Dimitrios, Vergkizi-Nikolakaki Souzan, Vizirianakis Ioannis S, Andriotis Eleftherios G, Eleftheriadis Georgios K, Fatouros Dimitrios G
Laboratory of Pharmaceutical Technology, Department of Pharmacy, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Digital Manufacturing and Materials Characterization Laboratory, School of Science and Technology, International Hellenic University, 57001 Thermi, Greece.
Pharmaceutics. 2022 Aug 5;14(8):1637. doi: 10.3390/pharmaceutics14081637.
In this study, drug carrier nanoparticles comprised of Pluronic-F127 and cannabidiol (CBD) or cannabigerol (CBG) were developed, and their wound healing action was studied. They were further incorporated in 3D printed films based on sodium alginate. The prepared films were characterized morphologically and physicochemically and used to evaluate the drug release profiles of the nanoparticles. Additional studies on their water loss rate, water retention capacity, and 3D-printing shape fidelity were performed. Nanoparticles were characterized physicochemically and for their drug loading performance. They were further assessed for their cytotoxicity (MTT Assay) and wound healing action (Cell Scratch Assay). The in vitro wound-healing study showed that the nanoparticles successfully enhanced wound healing in the first 6 h of application, but in the following 6 h they had an adverse effect. MTT assay studies revealed that in the first 24 h, a concentration of 0.1 mg/mL nanoparticles resulted in satisfactory cell viability, whereas CBG nanoparticles were safe even at 48 h. However, in higher concentrations and after a threshold of 24 h, the cell viability was significantly decreased. The results also presented mono-disperse nano-sized particles with diameters smaller than 200 nm with excellent release profiles and enhanced thermal stability. Their entrapment efficiency and drug loading properties were higher than 97%. The release profiles of the active pharmaceutical ingredients from the films revealed a complete release within 24 h. The fabricated 3D-printed films hold promise for wound healing applications; however, more studies are needed to further elucidate their mechanism of action.
在本研究中，制备了由普朗尼克-F127与大麻二酚（CBD）或大麻萜酚（CBG）组成的药物载体纳米颗粒，并研究了它们的伤口愈合作用。这些纳米颗粒进一步被掺入基于海藻酸钠的3D打印薄膜中。对制备的薄膜进行了形态学和物理化学表征，并用于评估纳米颗粒的药物释放曲线。还对其失水率、保水能力和3D打印形状保真度进行了额外研究。对纳米颗粒进行了物理化学表征及其载药性能研究。进一步评估了它们的细胞毒性（MTT法）和伤口愈合作用（细胞划痕试验）。体外伤口愈合研究表明，纳米颗粒在应用的前6小时成功促进了伤口愈合，但在接下来的6小时内产生了不利影响。MTT试验研究表明，在最初的24小时内，浓度为0.1mg/mL的纳米颗粒导致细胞活力令人满意，而CBG纳米颗粒即使在48小时时也是安全的。然而，在更高浓度以及超过24小时的阈值后，细胞活力显著下降。结果还呈现出直径小于200nm的单分散纳米颗粒，具有优异的释放曲线和增强的热稳定性。它们的包封率和载药性能高于97%。薄膜中活性药物成分的释放曲线显示在24小时内完全释放。制备的3D打印薄膜在伤口愈合应用方面具有前景；然而，需要更多研究来进一步阐明其作用机制。
