Sevilla Emma, Andreu Pilar, Fillat María F, Peleato M Luisa, Marín Juan A, Arbeloa Arancha
Department of Biochemistry, Molecular Biology, Institute of Biocomputation, Physics of Complex Systems, Universidad de Zaragoza, 50009 Zaragoza, Spain.
Pomology Department, Estación Experimental de Aula Dei CSIC, Av. Montañana 1005, 50059 Zaragoza, Spain.
Plants (Basel). 2022 Aug 12;11(16):2101. doi: 10.3390/plants11162101.
Fruit-tree rootstock selection is a challenge under a scenario of growing environmental stresses in which the soil and climate are greatly affected. Salinization is an increasing global process that severely affects soil fertility. The selection of rootstocks with the ability to tolerate salt stress is essential. Excised root cultures may be an excellent experimental approach to study stress physiology and a predictive tool to assess possible tolerance. In this study, we show how protein changes in response to salt stress evaluated in excised root cultures of (moderate salt-sensitive cultivar) could be representative of these changes in the roots of whole plants. The 2D electrophoresis of root extracts and subsequent spot identification by MALDI-TOF/TOF-MS show 16 relevant proteins differentially expressed in roots as a response to 60 mM NaCl. Cytoplasmic isozyme fructose 1,6-bisphosphate aldolase shows relevant changes in its relative presence of isoforms as a response to saline stress, while the total level of enzymes remains similar. Ferredoxin-NADP reductase increases as a response to salinity, even though the measured activity is not significantly different. The observed changes are congruent with previous proteomic studies on the roots of whole plants that are involved in protection mechanisms against salt stress.
在土壤和气候受到极大影响的环境压力不断增加的情况下，果树砧木的选择是一项挑战。盐渍化是一个日益全球化的过程，严重影响土壤肥力。选择具有耐盐胁迫能力的砧木至关重要。离体根培养可能是研究胁迫生理学的一种优秀实验方法，也是评估可能耐受性的一种预测工具。在本研究中，我们展示了在（中度盐敏感品种）的离体根培养中评估的响应盐胁迫的蛋白质变化如何能够代表整株植物根系中的这些变化。根提取物的二维电泳以及随后通过MALDI-TOF/TOF-MS进行的斑点鉴定显示，有16种相关蛋白质在根系中因60 mM NaCl而差异表达。细胞质同工酶果糖1,6-二磷酸醛缩酶在其同工型的相对存在方面表现出与盐胁迫相关的变化，而酶的总水平保持相似。铁氧还蛋白-NADP还原酶对盐度增加有响应，尽管测得的活性没有显著差异。观察到的变化与先前关于参与盐胁迫保护机制的整株植物根系的蛋白质组学研究一致。
