College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, No.120 Dongling Road, Shenhe District, Shenyang 110866, China; Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing 100101, China.
Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing 100101, China.
Comp Immunol Microbiol Infect Dis. 2021 Apr;75:101607. doi: 10.1016/j.cimid.2020.101607. Epub 2020 Dec 24.
Protozoans of Entamoeba spp. are globally distributed zoonotic parasites that infect diverse animal hosts and humans. Prevalence and species/genotypes distribution of Entamoeba spp. in domestic animals are not fully investigated on Qinghai-Tibetan Plateau (QTP), an animal husbandry and agriculture region of China. In a survey, 528 fecal samples were collected from 7 species of domestic animals on multiple locations across QTP region and analyzed by PCR and sequencing analysis. The overall prevalence of Entamoeba spp. infection in all examined animals was 97.9 %. Four Entamoeba species, E. bovis, E. moshkovskii, E. ecuadoriensis and E. histolytica were found, and majority (94.2 %) of Entamoeba-infected animals harbored E. bovis. Six Entamoeba genotypes, Entamoeba ribosomal lineages (RL) 1, 2, 3, 4, 8 and 9 were identified by sequencing analysis. Two zoonotic species, E. moshkovskii and E. histolytica, were present in horses, while E. ecuadoriensis and E. bovis were found in horses and all species of seven farm animals, respectively. It was also observed that six Entamoeba genotypes were distributed in animals in specific pattern. The results revealed high prevalence of Entamoeba spp. infection in livestock, broad range of hosts as well as diversity and species/genotype distribution of Entamoeba spp. in farm animals inhabiting on the high altitude QTP region.
原虫属的原虫分布广泛，是具有全球分布的动物源性寄生虫，感染多种动物宿主和人类。在青藏高原（QTP），即中国的畜牧业和农业区，对家畜中原虫属的流行情况和种/基因型分布尚未进行全面调查。在一项调查中，从 QTP 地区多个地点的 7 种家畜中采集了 528 份粪便样本，并通过 PCR 和测序分析进行了分析。所有检查动物中原虫属感染的总流行率为 97.9%。发现了 4 种原虫，即 E. bovis、E. moshkovskii、E. ecuadoriensis 和 E. histolytica，大多数（94.2%）感染原虫的动物携带 E. bovis。通过测序分析鉴定了 6 种原虫基因型，即核糖体谱系（RL）1、2、3、4、8 和 9。两种人畜共患种，E. moshkovskii 和 E. histolytica，存在于马中，而 E. ecuadoriensis 和 E. bovis 分别存在于马和 7 种家畜的所有物种中。还观察到 6 种原虫基因型在特定模式下分布在动物中。结果表明，家畜中原虫属感染的流行率很高，宿主范围广泛，以及栖息在高海拔 QTP 地区的家畜中原虫属的多样性和种/基因型分布。
