School of Biomedical Sciences, The Marshall Centre for Infectious Diseases Research and Training, The University of Western Australia, Crawley, WA, Australia.
Cooperative Research Centre for Honey Bee Products Limited (CRC HBP), The University of Western Australia, Crawley, WA, Australia.
BMC Complement Med Ther. 2022 Aug 26;22(1):228. doi: 10.1186/s12906-022-03695-x.
Honey has broad spectrum antibacterial activity against clinically important organisms and may be suitable for treating superficial bacterial infections. However, very little data are available describing potential interactions between honey and other topically applied agents such as antiseptics or essential oils.
Interactions between pairs of antibacterial agents were investigated by performing checkerboard assays and determining the fractional inhibitory concentration indices (FICIs). Interactions between the two monofloral honeys marri (from Corymbia calophylla) and manuka, and the antiseptic agents benzalkonium chloride, chlorhexidine digluconate, silver (I) nitrate, tea tree oil, and Eucalyptus polybractea oil were investigated against Staphylococcus aureus ATCC® 43300 and Pseudomonas aeruginosa ATCC® 27853.
Additive or indifferent interactions (FICI 0.5-2) were observed for all combinations against both organisms tested, with the exception of chlorhexidine and honey. Chlorhexidine and marri honey showed an antagonistic relationship against S. aureus (median FICI 2.00, range 1.25-4.83). Similarly, chlorhexidine and manuka honey showed antagonism against S. aureus (median FICI 2.33, range 2.00-2.67).
With the exception of chlorhexidine, these data indicate that honey does not interfere with the antimicrobial activity of the tested agents, and that honey may be suitable for combination therapy with other topically applied antibacterial agents for treating superficial bacterial infections.
蜂蜜对临床重要生物具有广谱抗菌活性，可能适合治疗浅表细菌感染。然而，描述蜂蜜与其他局部应用药物（如防腐剂或精油）之间潜在相互作用的可用数据非常有限。
通过进行棋盘微量稀释法测定和计算部分抑菌浓度指数（FICI）来研究抗菌药物对之间的相互作用。研究了两种单花蜜蜂蜜（来自 Corymbia calophylla 的马利和麦卢卡蜂蜜）和防腐剂苯扎氯铵、葡萄糖酸氯己定、硝酸银、茶树油和蓝桉油与金黄色葡萄球菌 ATCC® 43300 和铜绿假单胞菌 ATCC® 27853 之间的相互作用。
除了氯己定和蜂蜜外，所有组合对两种测试的生物体均表现出相加或无关（FICI 0.5-2）的相互作用。氯己定和马利蜂蜜对金黄色葡萄球菌表现出拮抗关系（中位数 FICI 为 2.00，范围为 1.25-4.83）。类似地，氯己定和麦卢卡蜂蜜对金黄色葡萄球菌也表现出拮抗作用（中位数 FICI 为 2.33，范围为 2.00-2.67）。
除氯己定外，这些数据表明蜂蜜不会干扰测试药物的抗菌活性，并且蜂蜜可能适合与其他局部应用的抗菌药物联合治疗浅表细菌感染。
