Lu Jian, Wang Weiguo, Zhang Cheng, Xu Wenping, Chen Weidong, Tao Liming, Li Zhong, Cheng Jiagao, Zhang Yang
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Department of Pathology, UT southwestern Medical Center, Dallas, TX 75390, United States.
Sci Total Environ. 2022 Dec 10;851(Pt 2):158308. doi: 10.1016/j.scitotenv.2022.158308. Epub 2022 Aug 27.
Glyphosate, the most widely used herbicide, presents new hazards to human health. The developmental toxicity of glyphosate, especially its cardiovascular toxicity, needs to be closely monitored. To understand how glyphosate affects development, we performed toxicity tests on zebrafish embryos that were continuously exposed to glyphosate. The results indicated that glyphosate affected the overall development of zebrafish embryos, including mortality, hatching abnormalities, and decreased body length. At the same time, zebrafish embryos exposed to glyphosate exhibited cardiac malformations, including enlarged chambers, thinned ventricular walls, and rhythm disturbances. In addition, defective intersegmental vasculature occurred after glyphosate exposure, indicating impaired angiogenesis. Mechanistically, apoptosis clustered in the heart and vascular regions and levels of ATP and apoptosis-related genes including caspase-3, caspase-9, bax, and bcl-2 were altered. In summary, the data showed that cardiovascular toxicity caused by glyphosate exposure may be related to apoptosis. Our study provides evidence for a link between glyphosate exposure and cardiovascular developmental toxicity. This raises concerns regarding the health risks of the glyphosate.
草甘膦是使用最广泛的除草剂，对人类健康构成新的危害。草甘膦的发育毒性，尤其是其心血管毒性，需要密切监测。为了解草甘膦如何影响发育，我们对持续暴露于草甘膦的斑马鱼胚胎进行了毒性测试。结果表明，草甘膦影响斑马鱼胚胎的整体发育，包括死亡率、孵化异常和体长缩短。同时，暴露于草甘膦的斑马鱼胚胎出现心脏畸形，包括心室扩大、心室壁变薄和节律紊乱。此外，草甘膦暴露后节段间血管系统出现缺陷，表明血管生成受损。从机制上讲，凋亡聚集在心脏和血管区域，并且三磷酸腺苷（ATP）水平以及包括半胱天冬酶-3、半胱天冬酶-9、bax和bcl-2在内的凋亡相关基因水平发生了改变。总之，数据表明草甘膦暴露引起的心血管毒性可能与凋亡有关。我们的研究为草甘膦暴露与心血管发育毒性之间的联系提供了证据。这引发了对草甘膦健康风险的担忧。
