Coppens Fanny, Willemarck Nicolas, Breyer Didier
Sciensano, Service Biosafety and Biotechnology, Brussels, Belgium.
Appl Biosaf. 2019 Dec 1;24(4):207-212. doi: 10.1177/1535676019871370.
While the European legislation states that laboratories of high-containment must be sealable for fumigation, they do not prescribe a minimal value for airtightness. Starting from a previous study in which we measured the airtightness in 4 BSL-3 laboratories with blower-door tests, we discuss the connection between airtightness and a successful decontamination by fumigation.
Biological indicators (BIs) consisting of spores of on metal disks were laid out in laboratories of different levels of airtightness before performing a fumigation with aerosolized hydrogen peroxide using an automated device, according to the manufacturer's instructions.
Incubation of all BI disks placed in the facility with the highest level of airtightness showed complete inactivation of spores. However, in the facility with a lower level of airtightness, not all spores were inactivated.
Air leaks might be a factor in the outcome of the decontamination of a room by fumigation, as seen in the laboratory with a lower level of airtightness, but other factors associated with the fumigation process might also be critical for a successful decontamination.
We argue that a validation of the decontamination procedure, before first use or after important renovations of a laboratory of high-containment, is a more effective endpoint than reaching a predefined level of airtightness.
虽然欧洲立法规定高防护实验室必须可密封以进行熏蒸，但并未规定气密性的最小值。基于之前一项我们通过鼓风门测试测量4个生物安全3级（BSL - 3）实验室气密性的研究，我们讨论气密性与熏蒸成功去污之间的联系。
按照制造商说明，在使用自动化设备对雾化过氧化氢进行熏蒸之前，将由金属盘上的孢子组成的生物指示剂（BIs）放置在不同气密性水平的实验室中。
放置在气密性最高的设施中的所有生物指示剂盘培养显示孢子完全失活。然而，在气密性较低的设施中，并非所有孢子都被灭活。
正如在气密性较低的实验室中所见，空气泄漏可能是熏蒸对房间去污效果的一个因素，但与熏蒸过程相关的其他因素对于成功去污也可能至关重要。
我们认为，在首次使用高防护实验室之前或进行重大翻新之后，对去污程序进行验证，比达到预定义的气密性水平是更有效的终点。
