Falaise Charlotte, Bouvattier Cécile, Larigauderie Guilhem, Lafontaine Valérie, Berchebru Laurent, Marangon Audrey, Vaude-Lauthier Valérie, Raynaud Françoise, Taysse Laurent
DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France.
Appl Biosaf. 2022 Mar 1;27(1):15-22. doi: 10.1089/apb.2021.0022. Epub 2022 Mar 15.
This study aimed to validate the efficacy of hydrogen peroxide vapor (HPV) decontamination technology set up in a biosafety level 3 (BSL-3) laboratory on surrogates and hazard group 3 (HG3) agents.
The HPV decontamination system (Bioquell) was assessed with both qualitative and quantitative methods on (1) spore surrogates (, , and ) in the BSL-3 laboratory and in the material airlock and on (2) HG3 agents ( SARS-CoV-2, Venezuelan equine encephalitis virus [VEE], and Vaccinia virus) in the BSL-3 laboratory. Other HG3 bacteria likely to be handled in the BSL-3 laboratory (, , , and ) were excluded from the HPV decontamination assays as preliminary viability tests demonstrated the total inactivation of these agents after 48 h drying on different materials.
The efficacy of HPV decontamination was validated with a reduction in viability of 5-7 log for the spores (surrogates and ), and for the enveloped RNA viruses. Vaccinia showed a higher resistance to the decontamination process, being dependent on the biological indicator location in the BSL-3 laboratory.
本研究旨在验证生物安全三级（BSL-3）实验室中建立的过氧化氢蒸汽（HPV）去污技术对替代物和第三类危险病原体（HG3）的有效性。
采用定性和定量方法对HPV去污系统（Bioquell）进行评估，评估对象包括：（1）BSL-3实验室和物料气闸中的孢子替代物（、和）；（2）BSL-3实验室中的HG3病原体（严重急性呼吸综合征冠状病毒2、委内瑞拉马脑炎病毒[VEE]和痘苗病毒）。由于初步生存能力测试表明，这些病原体在不同材料上干燥48小时后完全失活，因此HPV去污试验未纳入BSL-3实验室可能处理的其他HG3细菌（、、、和）。
HPV去污的有效性得到验证，孢子（替代物和）以及包膜RNA病毒的生存能力降低了5至7个对数。痘苗病毒对去污过程的抵抗力较高，这取决于其在BSL-3实验室中的生物指示剂位置。
