Ethanol and glycerol green emulsifying solvent for the formation of a essential oil/β-cyclodextrin inclusion complex: mixture design and adulticidal activity against .

The purpose of this study is to investigate the effect of essential oil medium on the inclusion complex of EO in β-cyclodextrin, as well as to examine the impact of the encapsulating action on the adulticidal activity. In line with this, EO was hydrodistilled and determined through GC-MS. Furthermore, the optimization of EO medium was conducted using a binary mixture design of ethanol and glycerol as green emulsifier solvent. Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction and thermogravimetric analysis were used to verify the establishment of the IC. The insecticidal effect of the created formulation was evaluated against female mosquitoes. The optimum ethanol: glycerol ratio was 0.73: 0.27, corresponding to 58.86% of encapsulation efficiency. The fumigant test showed that, after 24 h of exposure, EO exerted only 24.56 ± 1.04%, while the encapsulated oil killed 57.89% of the adult population. At the highest dose (312.5 μl/L), the encapsulated oil provided the most significant effect on adults (100% mortality after 54 h) compared to non-encapsulated oil (100% mortality after 72 h). The encapsulated form of EO constitutes a promising alternative for the control of mosquitoes that are responsible for human diseases.