Frey Greg, Robertson Cathy, Krishnan Jay
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
Appl Biosaf. 2020 Mar 1;25(1):48-52. doi: 10.1177/1535676019890975.
The objective of this study was to evaluate whether a Class II type A2 biosafety cabinet in a laboratory could be decontaminated while the laboratory was being fumigated using vaporous hydrogen peroxide or peracetic acid dry fogging.
To validate decontamination of all parts of the biosafety cabinet, biological indicators were placed at various locations within the biosafety cabinet, including between the pleats of supply and exhaust HEPA filters. To assess whether the operational status of the biosafety cabinet influenced the outcome of its decontamination, fumigation validations were undertaken with the cabinet running and not running. The amount of fumigant and the duration of fumigation remained constant whether the biosafety cabinet was running or not.
Biosafety cabinet decontamination was successful only when the cabinet was running to facilitate the fumigant's circulation within the plenums and across the HEPA filters. This study shows both vaporous hydrogen peroxide and peracetic acid dry fogging can be used successfully to decontaminate Class II type A2 biosafety cabinets during laboratory fumigation, provided the biosafety cabinets are operational and running during the fumigation.
本研究的目的是评估在使用气态过氧化氢或过氧乙酸干雾对实验室进行熏蒸时，实验室中的II级A2型生物安全柜是否能够被去污。
为验证生物安全柜所有部件的去污效果，将生物指示剂放置在生物安全柜内的不同位置，包括进风和排风高效空气过滤器的褶皱之间。为评估生物安全柜的运行状态是否会影响其去污结果，在生物安全柜运行和不运行的情况下进行熏蒸验证。无论生物安全柜是否运行，熏蒸剂的用量和熏蒸持续时间均保持不变。
只有当生物安全柜运行以促进熏蒸剂在静压箱内循环并穿过高效空气过滤器时，生物安全柜的去污才会成功。本研究表明，只要在熏蒸过程中生物安全柜处于运行状态，气态过氧化氢和过氧乙酸干雾均可成功用于对II级A2型生物安全柜进行去污。
