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用于分析斑马鱼时空心室收缩性和细胞核形态发生的尺度空间检测器

Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish.

作者信息

Teranikar Tanveer, Villarreal Cameron, Salehin Nabid, Ijaseun Toluwani, Lim Jessica, Dominguez Cynthia, Nguyen Vivian, Cao Hung, Chuong Cheng-Jen, Lee Juhyun

机构信息

Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA.

Martin High School/ UT Arlington, Arlington, TX, USA.

出版信息

iScience. 2022 Aug 5;25(9):104876. doi: 10.1016/j.isci.2022.104876. eCollection 2022 Sep 16.

DOI:10.1016/j.isci.2022.104876
PMID:36034231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9404658/
Abstract

quantitative assessment of structural and functional biomarkers is essential for characterizing the pathophysiology of congenital disorders. In this regard, fixed tissue analysis has offered revolutionary insights into the underlying cellular architecture. However, histological analysis faces major drawbacks with respect to lack of spatiotemporal sampling and tissue artifacts during sample preparation. This study demonstrates the potential of light sheet fluorescence microscopy (LSFM) as a non-invasive, 4D (3days + time) optical sectioning tool for revealing cardiac mechano-transduction in zebrafish. Furthermore, we have described the utility of a scale and size-invariant feature detector, for analyzing individual morphology of fused cardiomyocyte nuclei and characterizing zebrafish ventricular contractility.

摘要

对结构和功能生物标志物进行定量评估对于表征先天性疾病的病理生理学至关重要。在这方面，固定组织分析为潜在的细胞结构提供了革命性的见解。然而，组织学分析在样本制备过程中面临着缺乏时空采样和组织伪影的主要缺点。本研究证明了光片荧光显微镜（LSFM）作为一种非侵入性的4D（3天+时间）光学切片工具在揭示斑马鱼心脏机械转导方面的潜力。此外，我们还描述了一种尺度和大小不变特征检测器的效用，用于分析融合心肌细胞核的个体形态并表征斑马鱼心室收缩性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/dde733806116/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/7f252c9d42b2/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/5170df87fb2e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/c3df888caf7c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/ddfdf85ec639/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/749c62cef98c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/fe6b64e50e8a/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/dde733806116/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/7f252c9d42b2/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/5170df87fb2e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/c3df888caf7c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/ddfdf85ec639/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/749c62cef98c/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/fe6b64e50e8a/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290d/9404658/dde733806116/gr6.jpg

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Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.平面照明策略的发展，以解决亚细胞领域的奥秘。
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Zebrafish integrin a3b is required for cardiac contractility and cardiomyocyte proliferation.斑马鱼整合素 a3b 对于心脏收缩性和心肌细胞增殖是必需的。
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A novel method for tissue segmentation in high-resolution H&E-stained histopathological whole-slide images.一种用于高分辨率 H&E 染色组织学全切片图像组织分割的新方法。
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