Department of Hospital Infection Management, Sichuan Provincial Orthopedic Hospital, Chengdu, China.
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, (Chinese Center for Tropical Diseases Research); Key Laboratory of Parasite and Vector Biology, National Health Commission of People's Republic of China; National Center for International Research on Tropical Diseases, China; World Health Organization Collaborating Center for Tropical Diseases, Shanghai, China.
Front Cell Infect Microbiol. 2022 Aug 11;12:913393. doi: 10.3389/fcimb.2022.913393. eCollection 2022.
RNA-sequencing (RNA-seq) is a versatile, high-throughput technology that is being widely employed for screening differentially expressed genes (DEGs) in various diseases. Echinococcosis, a globally distributed zoonosis, has been reported to impose a heavy disease burden in pastoral areas of China. Herein we aimed to explore the molecular mechanisms underlying echinococcosis. In this study, peripheral blood samples were collected from six patients with alveolar echinococcosis (AE), six patients with cystic echinococcosis (CE), and six healthy controls. RNA-Seq (mRNA) was performed to detect gene transcript and expression levels, and DEGs were subjected to bioinformatic analyses. In comparison with healthy controls, 492 DEGs (270 upregulated, 222 downregulated) were found in the AE group and 424 DEGs (170 upregulated, 254 downregulated) were found in the CE group (|log (fold change)| > 1 and P < 0.05). Further, 60 genes were upregulated and 39 were downregulated in both the AE and CE groups. Gene ontology enrichment analysis indicated that DEGs were mainly involved in molecular functions, including extracellular space, extracellular region, organ and system development, and anatomical structure development. Protein-protein interaction (PPI) networks were constructed to depict the complex relationship between DEGs and interacting proteins.
RNA 测序（RNA-seq）是一种通用的高通量技术，广泛应用于筛选各种疾病中差异表达基因（DEGs）。包虫病是一种全球分布的动物源性传染病，在中国牧区造成了沉重的疾病负担。在此，我们旨在探讨包虫病的分子机制。本研究采集了 6 例泡型包虫病（AE）患者、6 例囊型包虫病（CE）患者和 6 例健康对照者的外周血样本。进行 RNA-Seq（mRNA）检测以检测基因转录物和表达水平，并对 DEGs 进行生物信息学分析。与健康对照组相比，AE 组有 492 个 DEGs（270 个上调，222 个下调），CE 组有 424 个 DEGs（170 个上调，254 个下调）（|log(fold change)|>1，P<0.05）。此外，AE 和 CE 两组中均有 60 个基因上调和 39 个基因下调。基因本体论富集分析表明，DEGs 主要参与分子功能，包括细胞外空间、细胞外区域、器官和系统发育以及解剖结构发育。构建蛋白质-蛋白质相互作用（PPI）网络以描绘 DEGs 与相互作用蛋白之间的复杂关系。
