Department of Infection and Immunology, Konkuk University School of Medicine, Seoul, Korea.
Department of Medicine, Jeju National University Graduate School, Jeju, Korea.
Int J Med Sci. 2022 Aug 8;19(9):1399-1407. doi: 10.7150/ijms.72565. eCollection 2022.
We hypothesized that the expression of exosomes under general anaesthesia with an inhalational anaesthetic agent would be changed. The study was designed to confirm the effect of general anesthesia with an inhalational anaesthetic agent on the expression of exosomes in rats. After intraperitoneal administration for the mixture of ketamine and xylazine, tracheal intubation was performed. Just before the connection to ventilator, Control group and Anaesthesia group, according to anaesthesia with isoflurane, were allocated. The expressions of exosomes were checked in bronchoalveolar lavage (BAL), the blood and the tissues from the lung and the brain. Cytokines in the blood were also assessed. The expressions of cluster of differentiation (CD)63 and CD81 as markers for the exosomes in the blood was increased after anaesthesia with isoflurane (CD63, 0.078 ± 0.057 % in Control group 0.180 ± 0.036 % in Anaesthesia group, = 0.02; CD81, 0.028 ± 0.034 % in Control group 0.245 ± 0.054 % in Anaesthesia group, < 0.01). However, the increased expression of them were not checked in BAL, and the tissues from the lung and the brain. The cytokines in the blood did not show any significant difference before and after anaesthesia with isoflurane. General anaesthesia with an inhalational anaesthetic agent increased the expression of exosomes in the blood. However, the change was limited in the blood, not the alveoli and the brain.
我们假设全身麻醉时吸入麻醉剂会改变外泌体的表达。本研究旨在证实吸入麻醉剂对大鼠外泌体表达的影响。在腹腔给予氯胺酮和二甲噻嗪混合物后，进行气管插管。就在连接呼吸机之前，根据异氟烷麻醉，将对照组和麻醉组进行分组。检查支气管肺泡灌洗液（BAL）、血液以及肺和脑组织中外泌体的表达。还评估了血液中的细胞因子。麻醉后血液中外泌体标志物 CD63 和 CD81 的表达增加（CD63，对照组为 0.078 ± 0.057%，麻醉组为 0.180 ± 0.036%， = 0.02；CD81，对照组为 0.028 ± 0.034%，麻醉组为 0.245 ± 0.054%， < 0.01）。然而，在 BAL 和肺及脑组织中并未检测到它们的表达增加。麻醉前后血液中的细胞因子没有显示出明显差异。吸入麻醉剂全身麻醉会增加血液中外泌体的表达。然而，这种变化仅限于血液，而不是肺泡和大脑。
