Department of Anesthesiology, Medical Faculty, University Hospital RWTH Aachen, 52074 Aachen, Germany.
Department of Intensive Care Medicine, Medical Faculty, University Hospital RWTH Aachen, 52074 Aachen, Germany.
Curr Issues Mol Biol. 2021 Nov 13;43(3):1997-2010. doi: 10.3390/cimb43030137.
Anesthetic-induced preconditioning (AIP) with volatile anesthetics is a well-known experimental technique to protect tissues from ischemic injury or oxidative stress. Additionally, plasmatic extracellular vesicle (EV) populations and their cargo are known to be affected by AIP in vitro, and to provide organ protective properties via their cargo. We investigated whether AIP would affect the generation of EVs in an in vivo rat model.
Twenty male Sprague Dawley rats received a repetitive treatment with either isoflurane or with sevoflurane for a duration of 4 or 8 weeks. EVs from blood plasma were characterized by nanoparticle tracking analysis, transmission electron microscopy (TEM) and Western blot. A scratch assay (H9C2 cardiomyoblast cell line) was performed to investigate the protective capabilities of the isolated EVs.
TEM images as well as Western blot analysis indicated that EVs were successfully isolated. The AIP changed the flotillin and CD63 expression on the EV surface, but not the EV concentration. The scratch assay did not show increased cell migration and/or proliferation after EV treatment.
AIP in rats changed the cargo of EVs but had no effect on EV concentration or cell migration/proliferation. Future studies are needed to investigate the cargo on a miRNA level and to investigate the properties of these EVs in additional functional experiments.
麻醉诱导预处理（AIP）用挥发性麻醉剂是一种众所周知的实验技术，可保护组织免受缺血性损伤或氧化应激。此外，已知血浆细胞外囊泡（EV）群体及其货物受 AIP 的影响，并通过其货物提供器官保护特性。我们研究了 AIP 是否会影响体内大鼠模型中 EV 的产生。
20 只雄性 Sprague Dawley 大鼠接受了重复的异氟烷或七氟烷治疗，持续 4 或 8 周。通过纳米颗粒跟踪分析、透射电子显微镜（TEM）和 Western blot 对来自血浆的 EV 进行了表征。划痕实验（H9C2 心肌细胞系）用于研究分离的 EV 的保护能力。
TEM 图像和 Western blot 分析表明成功分离了 EV。AIP 改变了 EV 表面的 flotillin 和 CD63 表达，但不改变 EV 浓度。划痕实验显示 EV 处理后细胞迁移和/或增殖没有增加。
AIP 在大鼠中改变了 EV 的货物，但对 EV 浓度或细胞迁移/增殖没有影响。未来的研究需要在 miRNA 水平上研究这些 EV 的货物，并在其他功能实验中研究这些 EV 的特性。
