Wakabayashi Naohiro, Yoshida Takumi, Oyama Kyohei, Naruse Daisuke, Tsutsui Masahiro, Kikuchi Yuta, Koga Daisuke, Kamiya Hiroyuki
Department of Cardiac Surgery, Asahikawa Medical University, Asahikawa, Japan.
Life Materials Development Section, Human Life Technology Research Institute, Toyama Industrial Technology Research and Development Center, Toyama, Japan.
Front Cardiovasc Med. 2022 Aug 11;9:946899. doi: 10.3389/fcvm.2022.946899. eCollection 2022.
The low patency of synthetic vascular grafts hinders their practical applicability. Polyvinyl alcohol (PVA) is a non-toxic, highly hydrophilic polymer; thus, we created a PVA-coated polycaprolactone (PCL) nanofiber vascular graft (PVA-PCL graft). In this study, we examine whether PVA could improve the hydrophilicity of PCL grafts and evaluate its performance using a rat aorta implantation model. A PCL graft with an inner diameter of 1 mm is created using electrospinning (control). The PCL nanofibers are coated with PVA, resulting in a PVA-PCL graft. Mechanical property tests demonstrate that the PVA coating significantly increases the stiffness and resilience of the PCL graft. The PVA-PCL surface exhibits a much smaller sessile drop contact angle when compared with that of the control, indicating that the PVA coating has hydrophilic properties. Additionally, the PVA-PCL graft shows significantly less platelet adsorption than the control. The proposed PVA-PCL graft is implanted into the rat's abdominal aorta, and its performance is tested at 8 weeks. The patency rate is 83.3% (10/12). The histological analysis demonstrates autologous cell engraftment on and inside the scaffold, as well as CD31/α-smooth muscle positive neointima regeneration on the graft lumen. Thus, the PVA-PCL grafts exhibit biocompatibility in the rat model, which suggests that the PVA coating is a promising approach for functionalizing PCL.
合成血管移植物的低通畅率阻碍了它们的实际应用。聚乙烯醇（PVA）是一种无毒、高度亲水的聚合物；因此，我们制备了一种聚乙烯醇涂层的聚己内酯（PCL）纳米纤维血管移植物（PVA-PCL移植物）。在本研究中，我们研究了PVA是否能提高PCL移植物的亲水性，并使用大鼠主动脉植入模型评估其性能。使用静电纺丝制备内径为1 mm的PCL移植物（对照）。PCL纳米纤维用PVA涂层，得到PVA-PCL移植物。力学性能测试表明，PVA涂层显著提高了PCL移植物的刚度和弹性。与对照相比，PVA-PCL表面的固着滴接触角小得多，表明PVA涂层具有亲水性。此外，PVA-PCL移植物的血小板吸附明显少于对照。将所提出的PVA-PCL移植物植入大鼠腹主动脉，并在8周时测试其性能。通畅率为83.3%（10/12）。组织学分析表明，支架上和支架内有自体细胞植入，移植物管腔内有CD31/α-平滑肌阳性新生内膜再生。因此，PVA-PCL移植物在大鼠模型中表现出生物相容性，这表明PVA涂层是使PCL功能化的一种有前景的方法。
