Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Guangzhou, China.
Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, China.
Food Funct. 2022 Sep 22;13(18):9674-9686. doi: 10.1039/d2fo01877b.
Triacylglycerol (TAG) is the primary constituent of human milk fat and plays a vital role in the healthy development of infants. But few studies reported the sophisticated profile of TAG molecular species in human breast milk and its temporal changes during a prolonged lactation period. An efficient ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) method was adopted to examine TAGs. A total of 128 TAGs in 296 human breast milk samples collected during postnatal 0 to 400 days were identified. The changes in the human milk TAG profile mainly took place in the early stages of lactation (postnatal 0-45 days), and the TAG profile became stable in mature milk after 200 days of lactation. Odd chain fatty acids (OC-FAs) may be important markers for identifying human breast milk of different lactation stages. This study could provide evidence for developing safe and efficacious human-milk substitutes for children without access to human breast milk.
三酰甘油（TAG）是人类乳脂的主要成分，在婴儿的健康发育中起着至关重要的作用。但很少有研究报道人乳中 TAG 分子种类的复杂特征及其在延长哺乳期内的时间变化。本研究采用高效超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF-MS）方法检测 TAG。共鉴定了 296 个人乳样本在产后 0 至 400 天期间的 128 种 TAG。人乳 TAG 谱的变化主要发生在哺乳期的早期（产后 0-45 天），200 天后的成熟乳中 TAG 谱变得稳定。奇数链脂肪酸（OC-FAs）可能是鉴定不同泌乳阶段人乳的重要标志物。本研究可为开发无法获得人乳的儿童安全有效的人乳替代物提供依据。
