Feng Jing Hui, Jung Jeon Sub, Hwang Seung Hwan, Lee Soo Kyeong, Lee Sang Youn, Kwak Youn Gil, Kim Doo-Ho, Song Chu-Youn, Kim Min Jung, Suh Hong Won, Kim Sung Chan, Lim Soon Sung
Department of Pharmacology, College of Medicine, Hallym University, Chuncheon, Republic of Korea.
Institute of Natural Medicine, College of Medicine, Hallym University, Chuncheon, Republic of Korea.
Anim Cells Syst (Seoul). 2022 Aug 8;26(4):166-173. doi: 10.1080/19768354.2022.2106302. eCollection 2022.
Pain and inflammation typically manifest in patients with arthritis. It is now widely known that Ledeb (AP) and Bunge (SM) exert anti-inflammatory and antinociceptive effects. We have previously reported that the mixture extract (ME) from AP and SM produces antinociceptive and anti-inflammatory effects in gout arthritis and monoiodoacetate (MIA)-induced arthritis models. In the present study, we assessed the antinociceptive and anti-inflammatory effects on the collagen-induced arthritis (CIA) model. The antinociceptive effects in mice were measured using the von Frey test. ME administered once or for one week (once per day) once, and one-week reduced the pain in a dose-dependent manner (from 50 to 100 mg/kg) in the CIA-induced osteoarthritis (OA) model. ME treatment also reduced tumor necrosis factor (TNF)-α and C-reactive protein (CRP) levels in plasma and ankle tissues. Furthermore, COX-1, COX-2, NF-κB, TNF-α, and IL-6 expressions were attenuated after ME treatment. In most experiments, the antinociceptive and anti-inflammatory effects induced by ME treatment were almost equal to or slightly better than those induced by (PC) treatment, which was used as a positive control. Our results suggest that ME possesses antinociceptive and anti-inflammatory effects, indicating its potential as a therapeutic agent for arthritis treatment.
疼痛和炎症通常在关节炎患者中表现出来。现在人们普遍知道，列当（AP）和远志（SM）具有抗炎和抗伤害感受作用。我们之前曾报道，AP和SM的混合提取物（ME）在痛风性关节炎和单碘乙酸（MIA）诱导的关节炎模型中产生抗伤害感受和抗炎作用。在本研究中，我们评估了其对胶原诱导的关节炎（CIA）模型的抗伤害感受和抗炎作用。使用von Frey试验测量小鼠的抗伤害感受作用。在CIA诱导的骨关节炎（OA）模型中，ME单次给药或连续一周（每天一次）给药，剂量为50至100mg/kg时，能以剂量依赖的方式减轻疼痛。ME治疗还降低了血浆和踝关节组织中肿瘤坏死因子（TNF）-α和C反应蛋白（CRP）的水平。此外，ME治疗后COX-1、COX-2、NF-κB、TNF-α和IL-6的表达减弱。在大多数实验中，ME治疗诱导的抗伤害感受和抗炎作用几乎等同于或略优于用作阳性对照的（PC）治疗诱导的作用。我们的结果表明，ME具有抗伤害感受和抗炎作用，表明其作为关节炎治疗药物的潜力。
