Jang Hwan Hee, Nam Song Yee, Kim Mi Ju, Kim Jung Bong, Choi Jeong Sook, Kim Haeng Ran, Lee Young Min
Functional Food & Nutrition Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, Republic of Korea.
Division of Applied Food System, Major of Food and Nutrition, Seoul Women's University, Seoul, 01797, South Korea.
BMC Complement Altern Med. 2017 Sep 5;17(1):442. doi: 10.1186/s12906-017-1949-z.
Agrimonia pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. pilosa on impaired glucose metabolism induced by a high-fat diet in rats.
Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis.
The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p < 0.05); however, supplementation with A. pilosa decreased liver weight. Blood glucose levels in the HFA group were lower than levels measured in the HF group 30, 60, and 120 min after glucose administration (p < 0.05). In addition, dietary A. pilosa supplementation decreased tumor necrosis factor α and interleukin 6 levels, while increasing serum adiponectin concentrations (p < 0.05 vs. the HF group). These effects were accompanied by reduced hepatic and adipose tissue expression of inflammation-related genes such as Tnf and Il1b (p < 0.05).
Our findings indicate that A. pilosa aqueous extract can ameliorate insulin resistance in high-fat diet-fed rats by decreasing the inflammatory response.
龙牙草是一种具有抗癌、抗氧化、抗炎活性以及体外抗糖尿病活性等生理活性的药用植物。然而，龙牙草水提取物对胰岛素抵抗大鼠的影响尚未得到研究。我们研究了龙牙草水提取物对高脂饮食诱导的大鼠葡萄糖代谢受损的影响。
将雄性Sprague-Dawley大鼠分为以下几组：正常脂肪饮食组（NF，n = 9）；高脂饮食组（HF，n = 9）；高脂饮食+0.1%龙牙草水提取物组（HFA，n = 10）。给予实验性饮食16周。在治疗结束时，分离肝脏和脂肪组织，并收集血清进行生化分析。
HF组大鼠的肝脏重量显著高于NF组大鼠，且肝脏脂质积累增加（p < 0.05）；然而，补充龙牙草可降低肝脏重量。给予葡萄糖后30、60和120分钟，HFA组的血糖水平低于HF组（p < 0.05）。此外，饮食中补充龙牙草可降低肿瘤坏死因子α和白细胞介素6水平，同时增加血清脂联素浓度（与HF组相比，p < 0.05）。这些作用伴随着肝脏和脂肪组织中炎症相关基因如Tnf和Il1b的表达降低（p < 0.05）。
我们的研究结果表明，龙牙草水提取物可通过减轻炎症反应改善高脂饮食喂养大鼠的胰岛素抵抗。
