People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, Ningxia, China.
Ningxia Medical University, Yinchuan, Ningxia, China.
Biomed Res Int. 2022 Aug 21;2022:3727165. doi: 10.1155/2022/3727165. eCollection 2022.
Noncoding RNAs were discovered to control a variety of developmental mechanisms, including osteogenesis. According to emerging evidence, cryptochrome circadian-regulating (CRY) proteins have emerged as essential controllers of osteoblast differentiation. The linked processes, on the other hand, are still unknown. The specific process that underpins osteoblast differentiation and proliferation is yet unknown. This research gives a meta-analysis of CRY2's impact on mouse osteoblast differentiation via the control of the WNT/-catenin signaling pathways. Western blot and quantitative real-time PCR were used to identify Cry2 expression levels, components in the osteoblast-associated signaling pathway, and osteoblast transcription markers. The osteogenic condition was measured utilizing alkaline phosphatase (ALP) and alizarin red (AR) staining, whereas cell growth rates were measured using CCK8 assays. An ectopic bone formation experiment was used to determine the osteogenic potential of osteoblasts. Cry2 stimulates the osteogenic development of mouse osteoblasts through canonical Wnt/-catenin signaling, according to the findings.
非编码 RNA 被发现可控制多种发育机制，包括成骨作用。根据新出现的证据，隐花色素生物钟调节（CRY）蛋白已成为成骨细胞分化的重要控制器。另一方面，相关的过程仍不清楚。支持成骨细胞分化和增殖的具体过程尚不清楚。本研究通过控制 WNT/-catenin 信号通路，对 CRY2 对小鼠成骨细胞分化的影响进行了荟萃分析。采用 Western blot 和实时定量 PCR 检测 Cry2 表达水平、成骨细胞相关信号通路的组成部分和成骨细胞转录标志物。利用碱性磷酸酶（ALP）和茜素红（AR）染色检测成骨条件，而利用 CCK8 测定细胞生长速率。通过异位骨形成实验确定成骨细胞的成骨潜能。研究结果表明，Cry2 通过经典 Wnt/-catenin 信号刺激小鼠成骨细胞的成骨发育。
