Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012 Paris, France.
Double Helix Optics Inc., Boulder, CO, USA.
Cell Rep Methods. 2022 Aug 15;2(8):100268. doi: 10.1016/j.crmeth.2022.100268. eCollection 2022 Aug 22.
We developed a multi-unit microscope for all-optical inter-layers circuits interrogation. The system performs two-photon (2P) functional imaging and 2P multiplexed holographic optogenetics at axially distinct planes. We demonstrated the capability of the system to map, in the mouse retina, the functional connectivity between rod bipolar cells (RBCs) and ganglion cells (GCs) by activating single or defined groups of RBCs while recording the evoked response in the GC layer with cell-type specificity and single-cell resolution. We then used a logistic model to probe the functional connectivity between cell types by deriving the "cellular receptive field" describing how RBCs impact each GC type. With the capability to simultaneously image and control neuronal activity at axially distinct planes, the system enables a precise interrogation of multi-layered circuits. Understanding this information transfer is a promising avenue to dissect complex neural circuits and understand the neural basis of computations.
我们开发了一种多单元显微镜，用于全光学层间电路检测。该系统在轴向不同的平面上执行双光子（2P）功能成像和 2P 复用全息光遗传学。我们展示了该系统在小鼠视网膜中通过激活单个或特定的 RBC 组，同时以细胞类型特异性和单细胞分辨率记录 GC 层中的诱发电响应，来绘制 RBC 与 GC 之间功能连接的能力。然后，我们使用逻辑模型通过推导出描述 RBC 如何影响每种 GC 类型的“细胞感受野”来探测细胞类型之间的功能连接。该系统具有在轴向不同的平面上同时成像和控制神经元活动的能力，能够精确检测多层电路。理解这种信息传递是剖析复杂神经回路并理解计算神经基础的有前途的途径。
