Institute of Applied Biology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
Physiol Res. 2022 Nov 28;71(5):713-717. doi: 10.33549/physiolres.934881. Epub 2022 Aug 31.
The aim of this study was to evaluate the impact of diabetes mellitus type 2 (DM2) on the male endocrine system of Zucker Diabetic Fatty (ZDF) rats. Sexually mature ZDF rats were divided to a lean (control) and obese group, and had diabetes confirmed by blood tests. For the in vivo experiment, fasting blood was collected to obtain blood plasma. In case of the in vitro experiments, testicular fragments were cultured for 24 h, and the culture medium was collected. The concentrations of testosterone (T), androstenedione (A4), dehydroepiandrosterone (DHEA-S), estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were quantified in the blood plasma and the medium by the ELISA method, while cholesterol (CHOL) was assessed spectrophotometrically. A significant decline of T (36.31 %), A4 (25.11 %) and FSH (26.99 %) as well as a significant increase of CHOL and E2 (36.17 %) was observed in the blood plasma of obese ZDF rats in comparison to the control. Under in vitro conditions, a significant decrease of FSH (23.35 %) accompanied by an increase of E2 was observed in the obese group compared to the control. In the case of CHOL, LH, T, DHEA and A4 no significant differences were observed. Our results suggest that except for FSH and E2 all steroid biomolecules were synthetized normally by the testicular tissue, however a dramatic endocrine disturbance was observed at the system level. We may conclude that DM2 has negative effects on systemic hormone secretion and these alterations are more pronounced in combination with obesity.
本研究旨在评估 2 型糖尿病（DM2）对 Zucker 糖尿病肥胖（ZDF）大鼠雄性内分泌系统的影响。成熟的 ZDF 大鼠被分为瘦（对照）和肥胖组，并通过血液检查确认患有糖尿病。对于体内实验，采集空腹血液以获得血浆。对于体外实验，将睾丸组织片段培养 24 小时，并收集培养介质。通过 ELISA 方法定量检测血浆和培养基中睾酮（T）、雄烯二酮（A4）、脱氢表雄酮（DHEA-S）、雌二醇（E2）、卵泡刺激素（FSH）和黄体生成素（LH）的浓度，同时使用分光光度法评估胆固醇（CHOL）。与对照组相比，肥胖 ZDF 大鼠的血浆中 T（36.31%）、A4（25.11%）和 FSH（26.99%）显著下降，而 CHOL 和 E2 显著增加（36.17%）。在体外条件下，与对照组相比，肥胖组的 FSH（23.35%）显著下降，同时 E2 增加。而 CHOL、LH、T、DHEA 和 A4 则没有明显差异。我们的结果表明，除了 FSH 和 E2，睾丸组织正常合成所有类固醇生物分子，但在系统水平上观察到明显的内分泌紊乱。我们可以得出结论，DM2 对全身激素分泌有负面影响，而这些改变在与肥胖结合时更为明显。
