冷冻电镜在探索 RNA 结构动态方面的前景。
The promise of cryo-EM to explore RNA structural dynamics.
机构信息
Department of Biochemistry and Molecular Genetics, Aurora, CO 80045, USA. Electronic address: https://twitter.com/Steve_Bonilla.
Department of Biochemistry and Molecular Genetics, Aurora, CO 80045, USA; RNA BioScience Initiative, University of Colorado Anschutz Medical Campus, School of Medicine, Aurora, CO 80045, USA.
出版信息
J Mol Biol. 2022 Sep 30;434(18):167802. doi: 10.1016/j.jmb.2022.167802. Epub 2022 Aug 29.
Conformational dynamics are essential to macromolecular function. This is certainly true of RNA, whose ability to undergo programmed conformational dynamics is essential to create and regulate complex biological processes. However, methods to easily and simultaneously interrogate both the structure and conformational dynamics of fully functional RNAs in isolation and in complex with proteins have not historically been available. Due to its ability to image and classify single particles, cryogenic electron microscopy (cryo-EM) has the potential to address this gap and may be particularly amenable to exploring structural dynamics within the three-dimensional folds of biologically active RNAs. We discuss the possibilities and current limitations of applying cryo-EM to simultaneously study RNA structure and conformational dynamics, and present one example that illustrates this (as of yet) not fully realized potential.
构象动力学对于生物大分子的功能至关重要。这对于 RNA 来说尤其如此，因为其能够进行程序性构象动力学是创建和调节复杂生物过程的关键。然而，在过去，并没有一种简单而又能同时检测完整功能的 RNA 结构及其构象动力学的方法，无论是在单独状态还是与蛋白质结合状态下都是如此。由于 cryo-EM（低温电子显微镜）具有成像和分类单个颗粒的能力，因此它有潜力解决这一差距，并且可能特别适合于探索生物活性 RNA 的三维折叠结构内的结构动力学。我们讨论了将 cryo-EM 应用于同时研究 RNA 结构和构象动力学的可能性和当前限制，并提供了一个实例来说明这一（目前）尚未完全实现的潜力。
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