Department of Applied Biology, C. K. Tedam University of Technology and Applied Sciences, Navrongo, Ghana.
Directorate of Plant Protection, Quarantine and Storage, Faridabad, Haryana, India.
Mol Biol Rep. 2024 Jul 11;51(1):791. doi: 10.1007/s11033-024-09755-4.
Heat stress is a detrimental abiotic stress that limits the development of many plant species and is linked to a variety of cellular and physiological problems. Heat stress affects membrane fluidity, which leads to negative effects on cell permeability and ion transport. Research reveals that heat stress causes severe damage to cells and leads to rapid accumulation of reactive oxygen species (ROS), which could cause programmed cell death.
This current study aimed to validate the role of Triticum aestivum Salt Stress Root Protein (TaSSRP) in plants' tolerance to heat stress by modulating its expression in tobacco plants. The Relative Water Content (RWC), total chlorophyll content, and Membrane Stability Index (MSI) of the seven distinct transgenic lines (T, T, T, T, T, T, and T), increased in response to heat stress. Despite the fact that the same tendency was detected in wild-type (WT) plants, changes in physio-biochemical parameters were greater in transgenic lines than in WT plants. The expression analysis revealed that the transgene TaSSRP expressed from 1.00 to 1.809 folds in different lines in the transgenic tobacco plants. The gene TaSSRP offered resistance to heat stress in Nicotiana tabacum, according to the results of the study.
These findings could help to improve our knowledge and understanding of the mechanism underlying thermotolerance in wheat, and the novel identified gene TaSSRP could be used in generating wheat varieties with enhanced tolerance to heat stress.
热应激是一种有害的非生物胁迫，限制了许多植物物种的发育，并与多种细胞和生理问题有关。热应激会影响膜的流动性，从而对细胞通透性和离子运输产生负面影响。研究表明，热应激会对细胞造成严重损伤，并导致活性氧（ROS）的快速积累，从而可能导致细胞程序性死亡。
本研究旨在通过在烟草植物中调节 TaSSRP 的表达来验证其在植物耐热性中的作用。7 个不同的转基因系（T1、T2、T3、T4、T5、T6 和 T7）的相对水含量（RWC）、总叶绿素含量和膜稳定性指数（MSI）在受到热应激时增加。尽管在野生型（WT）植物中也检测到了相同的趋势，但转基因系中生理生化参数的变化大于 WT 植物。表达分析表明，转 TaSSRP 基因在不同的转基因烟草系中表达水平为 1.00 到 1.809 倍。研究结果表明，TaSSRP 基因赋予了烟草对热应激的抗性。
这些发现有助于提高我们对小麦耐热性机制的认识和理解，新鉴定的 TaSSRP 基因可用于培育对热应激具有更高耐受性的小麦品种。
