Department of Ophthalmology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai 201102, China.
Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Biomed Res Int. 2022 Aug 23;2022:4740141. doi: 10.1155/2022/4740141. eCollection 2022.
To identify the potential key genes and molecular pathways associated with keratoconus and allergic disease.
The pubmed2ensembl database was used to identify the text mining genes (TMGs) collectively involved in keratoconus and allergic disease. The GeneCodis program was used to perform the Gene Ontology (GO) biological process and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of TMGs. The protein-protein interaction (PPI) network of the TMGs was established by STRING; the significant gene modules and hub genes of PPI were further performed using the Cytoscape software. The DAVID database was used to perform the GO and KEGG analyses of the significant module.
In total, 98 TMGs collectively involved in keratoconus and allergic disease were identified. 19 enriched biological processes including 71 genes and 25 enriched KEGG pathways including 59 genes were obtained. A TMG PPI network was constructed, and 51 genes/nodes were identified with 110 edges; 3 most significant modules and 12 hub genes were chosen from the PPIs. GO enrichment analysis showed that the TMGs were primarily associated with collagen catabolic process, extracellular matrix organization and disassembly, cell adhesion and migration, collagen-containing extracellular matrix, extracellular matrix, and structure organization. KEGG pathway analysis showed that these DEGs were mainly involved in the IL-17 signaling pathway, inflammatory bowel disease, rheumatoid arthritis, allograft rejection, T cell receptor signaling pathway, cytokine-cytokine receptor interaction, and TNF signaling pathway.
The results revealed that , , , , , , , , , , , and were potential key genes involved in keratoconus. IL-17 signaling pathway was the potential pathways accounting for pathogenesis and development of keratoconus.
鉴定与圆锥角膜和过敏性疾病相关的潜在关键基因和分子途径。
使用 pubmed2ensembl 数据库鉴定共同涉及圆锥角膜和过敏性疾病的文本挖掘基因（TMG）。使用 GeneCodis 程序对 TMG 进行基因本体论（GO）生物过程和京都基因与基因组百科全书（KEGG）途径富集分析。通过 STRING 建立 TMG 的蛋白质-蛋白质相互作用（PPI）网络；进一步使用 Cytoscape 软件对 PPI 的显著基因模块和枢纽基因进行分析。使用 DAVID 数据库对显著模块进行 GO 和 KEGG 分析。
共鉴定出 98 个共同涉及圆锥角膜和过敏性疾病的 TMG。获得了 19 个富集的生物过程，包括 71 个基因，和 25 个富集的 KEGG 途径，包括 59 个基因。构建了一个 TMG PPI 网络，鉴定出 51 个基因/节点和 110 个边；从 PPI 中选择了 3 个最重要的模块和 12 个枢纽基因。GO 富集分析表明，TMG 主要与胶原分解代谢过程、细胞外基质组织和分解、细胞黏附和迁移、含有胶原的细胞外基质、细胞外基质和结构组织有关。KEGG 途径分析表明，这些差异表达基因主要参与白细胞介素 17 信号通路、炎症性肠病、类风湿关节炎、同种异体移植排斥、T 细胞受体信号通路、细胞因子-细胞因子受体相互作用和 TNF 信号通路。
结果表明 、 、 、 、 、 、 、 、 、 是参与圆锥角膜的潜在关键基因。白细胞介素 17 信号通路是解释圆锥角膜发病机制和发展的潜在途径。
