Whole-genome sequence of a high-temperature edible mushroom (zhudugu).

Most of the sequenced wood-rotting edible mushroom produce fruiting body at relatively low temperatures. Little information has been known about the high-temperature wood-rotting mushroom. Here, we performed sequencing and assembly of the genome of a high-temperature edible mushroom from a monokaryotic strain zhudugu2 using the Illumina and Pac-Bio CLR sequencing technologies. , also known as Zhudugu in China, is a well-known culinary edible mushroom that has been widely distributed and cultivated in China, Southeast Asia, and South Asia. The genome consists of 40.00 Mb in 27 contigs with a contig N50 of 4.384 Mb. Phylogenetic analysis reveals that and other strains in clustered in one clade. Phylogenetic analysis and average nucleotide identity analysis indicated that the genome showed a closer relationship with other species. Chromosome collinearity analysis revealed a high level of collinearity between and . There are 12,628 protein-coding genes annotated in this monoploid genome. A total of 481 enzymes accounting for 514 carbohydrate-active enzymes (CAZymes) terms were identified in the genome, including 15 laccases and 10 class II peroxidases predicted in the genome, which revealed the robustness of lignocellulose degradation capacity of . The mating- type locus of consisted of a pair of homeodomain mating-type genes and . The mating- type locus of consisted of at least four pheromone receptor genes and three pheromone genes. The genome is not only beneficial for the genome-assisted breeding of this mushroom but also helps us to understand the high-temperature tolerance of the edible mushroom.