Sadler T W, Hunter E S
Am J Obstet Gynecol. 1987 Jul;157(1):190-3. doi: 10.1016/s0002-9378(87)80378-2.
The effects of hypoglycemia on mammalian embryos undergoing neurulation (third to fourth week of human development) were investigated. Mouse embryos were maintained for 28 hours in whole embryo culture in serum collected from rats that had received 50 units of 100 United States Pharmacopeia insulin units per milliliter. Glucose concentrations used were 40, 60, 80, and 147 mg/dl (normal blood glucose in the pregnant mouse is 125 mg/dl). After the culture period embryos were evaluated for malformations and growth and compared with those maintained under euglycemic conditions. The results demonstrate that glucose concentrations approximately 50% of normal maternal levels were teratogenic but not growth inhibitory, whereas concentrations in the range of 30% to 40% of maternal levels were lethal to the embryo. Furthermore, a 14-hour exposure to reduced blood sugar in either the first or second half of the culture period produced malformations.
研究了低血糖对处于神经胚形成阶段（人类发育的第三至第四周）的哺乳动物胚胎的影响。将小鼠胚胎在从接受每毫升含50单位100美国药典胰岛素单位的大鼠采集的血清中进行全胚胎培养28小时。使用的葡萄糖浓度分别为40、60、80和147毫克/分升（怀孕小鼠的正常血糖为125毫克/分升）。培养期结束后，对胚胎进行畸形和生长评估，并与在正常血糖条件下培养的胚胎进行比较。结果表明，约为正常母体水平50%的葡萄糖浓度具有致畸性但不抑制生长，而在母体水平30%至40%范围内的浓度对胚胎是致命的。此外，在培养期的前半段或后半段，胚胎暴露于低血糖环境14小时会导致畸形。
