Department of Ophthalmology, NYU Langone Health, New York, NY, USA.
Department of Radiology, NYU Langone Health, New York, NY, USA.
Transl Vis Sci Technol. 2022 Sep 1;11(9):3. doi: 10.1167/tvst.11.9.3.
We employ visible light optical coherence tomography (OCT) to investigate the relationship between the myoid, ellipsoid, and band 2 in the living human retina. Rather than refute existing theories, we aim to reveal new bands and better delineate the structures at hand.
An upgraded spectral/Fourier domain visible light OCT prototype, with 1.0-µm axial resolution, imaged 13 eyes of 13 young adult human subjects (23-40 years old) without a history of ocular pathology. The external limiting membrane (band 1) and band 2 edges were segmented. Reflectivity was examined along the inner segment (IS), defined as extending from band 1 to the band 2 center, and within band 2 itself.
Images highlight a nearly continuously resolved extrafoveal internal limiting membrane, the peripheral single-cell thick ganglion cell layer, and the peripheral photoreceptor axonal fiber layer, a peripheral division of band 2 into bands 2a and 2b, and a reflectivity-based division of the IS into "m" and "e" zones.
Topography and transverse intensity variations of the outermost band 2b suggest an association with rods. The "m" and "e" zone border is consistent with the myoid-ellipsoid boundary, even recapitulating the well-documented distribution of mitochondria throughout the IS at the foveal center. Theories of outer retinal reflectivity in OCT must adequately explain these observations.
Findings support that band 2 does partially overlap with the ellipsoid in transversally averaged OCT images due to photoreceptor IS length dispersion but argue that the inner ellipsoid must be inner to band 2, as suggested by prior quantitative measurements.
我们利用可见光光学相干断层扫描（OCT）来研究活体人视网膜中的肌样体、椭圆体和带 2 之间的关系。我们的目的不是反驳现有的理论，而是揭示新的带并更好地描绘手头的结构。
使用具有 1.0μm 轴向分辨率的升级光谱/傅里叶域可见光 OCT 原型，对 13 名年轻成年人类受试者（23-40 岁）的 13 只眼睛进行成像，这些受试者没有眼部病理学病史。分割外部限制膜（带 1）和带 2 边缘。在内部节段（IS）内检查反射率，定义为从带 1 延伸到带 2 中心，并在带 2 本身内。
图像突出显示了一个几乎连续分辨的周边外部限制膜、周边单细胞厚的节细胞层和周边光感受器轴突纤维层，带 2 被分为带 2a 和带 2b 的带 2 周边划分，以及基于反射率的 IS 分为“m”和“e”区。
最外层带 2b 的形貌和横向强度变化表明与视杆有关。“m”和“e”区边界与肌样体-椭圆体边界一致，甚至再现了在黄斑中心整个 IS 中记录良好的线粒体分布。OCT 中外视网膜反射率的理论必须充分解释这些观察结果。
目的：我们利用可见光光学相干断层扫描（OCT）来研究活体人视网膜中的肌样体、椭圆体和带 2 之间的关系。我们的目的不是反驳现有的理论，而是揭示新的带并更好地描绘手头的结构。
使用具有 1.0μm 轴向分辨率的升级光谱/傅里叶域可见光 OCT 原型，对 13 名年轻成年人类受试者（23-40 岁）的 13 只眼睛进行成像，这些受试者没有眼部病理学病史。分割外部限制膜（带 1）和带 2 边缘。在内部节段（IS）内检查反射率，定义为从带 1 延伸到带 2 中心，并在带 2 本身内。
图像突出显示了一个几乎连续分辨的周边外部限制膜、周边单细胞厚的节细胞层和周边光感受器轴突纤维层，带 2 被分为带 2a 和带 2b 的带 2 周边划分，以及基于反射率的 IS 分为“m”和“e”区。
最外层带 2b 的形貌和横向强度变化表明与视杆有关。“m”和“e”区边界与肌样体-椭圆体边界一致，甚至再现了在黄斑中心整个 IS 中记录良好的线粒体分布。OCT 中外视网膜反射率的理论必须充分解释这些观察结果。
带 2 确实部分重叠在 OCT 横断面上的椭圆体，这是由于光感受器 IS 长度的分散，但这表明内椭圆体必须在带 2 内，如先前的定量测量所建议的。
