Kanarek R B, Aprille J R, Hirsch E, Gualtiere L, Brown C A
Am J Physiol. 1987 Jul;253(1 Pt 2):R158-66. doi: 10.1152/ajpregu.1987.253.1.R158.
Adult male Sprague-Dawley rats were divided into three groups and fed diets containing either 10, 20, or 40% protein for 56 days. Half of the rats in each dietary condition were given a 32% sucrose solution plus the standard diet and water. Sucrose intake varied directly as a function of dietary protein levels. Rats fed either the 10 or 20% protein diet and sucrose had higher caloric intakes, gained more weight, were more efficient at using calories for weight gain, and had more adipose tissue than rats given the same diet without sucrose. Rats fed the 40% protein diet and sucrose did not exhibit overeating, excess weight gain, or increased feed efficiency relative to animals fed the 40% diet alone. Animals given sucrose had more interscapular brown adipose tissue (IBAT) and a greater metabolic potential for thermogenesis in IBAT as determined by GDP binding in mitochondria than rats not fed sucrose. These results demonstrate that dietary protein is important in the development of sucrose-induced obesity and that increases in IBAT mass and activity can occur concomitant with increased feed efficiency.
成年雄性斯普拉格-道利大鼠被分为三组，分别喂食含10%、20%或40%蛋白质的饲料，持续56天。每种饮食条件下的一半大鼠给予32%的蔗糖溶液以及标准饲料和水。蔗糖摄入量直接随饮食蛋白质水平而变化。喂食含10%或20%蛋白质饲料及蔗糖的大鼠比喂食相同饲料但不含蔗糖的大鼠热量摄入更高、体重增加更多、利用热量增加体重的效率更高且脂肪组织更多。相对于仅喂食40%蛋白质饲料的动物，喂食40%蛋白质饲料及蔗糖的大鼠未表现出暴饮暴食、体重过度增加或饲料效率提高。与未喂食蔗糖的大鼠相比，喂食蔗糖的动物肩胛间棕色脂肪组织（IBAT）更多，且通过线粒体中GDP结合测定，IBAT的产热代谢潜力更大。这些结果表明，饮食蛋白质在蔗糖诱导的肥胖发展中很重要，并且IBAT质量和活性的增加可能与饲料效率提高同时发生。
