Flamme I
Anat Embryol (Berl). 1987;176(1):45-52. doi: 10.1007/BF00309751.
The method described in this paper allows explanted chick embryos to develop normally in vitro from the 2nd to the 5th day of incubation. The embryos are explanted with the aid of a ring of filter-paper and are fixed between two steel-rings dorsal side up for cultivation. The lower ring contains a mixture of yolk and albumen as culture medium. 40% of the explanted embryos reach the 5th day of development (stage 25, Hamburger and Hamilton). The maximum time of culture is 70 h after explantation.
本文所述方法可使移植的鸡胚在体外从孵化的第2天到第5天正常发育。借助一圈滤纸将胚胎取出，并将其背侧朝上固定在两个钢环之间进行培养。下面的钢环装有蛋黄和蛋白的混合物作为培养基。40%的移植胚胎发育到第5天（哈默伯格和汉密尔顿分期的第25期）。培养的最长时间是移植后70小时。
