Naito M, Nirasawa K, Oishi T
National Institute of Animal Industry, Tsukuba Norindanchi, Ibaraki, Japan.
J Exp Zool. 1990 Jun;254(3):322-6. doi: 10.1002/jez.1402540311.
An experiment was carried out to investigate whether thick albumen is essential for the normal development of the chick embryo. Fertilized ova recovered from the oviducts of hens were cultured in vitro and transferred to recipient eggshells with (method A) or without (method B) replacement of the thick albumen by thin albumen. Embryos from freshly laid eggs were transferred to recipient eggshells with (method C) or without (method E) replacement of the thick albumen by thin albumen or with replacement of the thick albumen by thin albumen diluted with solution of salts (method D). Embryos were then incubated until hatching. The rates of hatching of the cultured embryos were 34.4%, 16.2%, 50.0%, 6.9-26.7%, and 47.5% for methods A, B, C, D, and E, respectively. Thus the rate of hatching of cultured fertilized ova was increased by replacement of the thick albumen by thin albumen at the blastoderm stage. Chicks obtained by method A reached maturity and produced viable offspring, and this technique provides an improved method for the culture of fertilized ova to hatching.
进行了一项实验，以研究浓稠蛋白对鸡胚正常发育是否至关重要。从母鸡输卵管中回收的受精卵在体外培养，然后转移到接受者蛋壳中，其中浓稠蛋白被稀蛋白替代（方法A）或未被替代（方法B）。将刚产下的鸡蛋中的胚胎转移到接受者蛋壳中，其中浓稠蛋白被稀蛋白替代（方法C）或未被替代（方法E），或者浓稠蛋白被用盐溶液稀释的稀蛋白替代（方法D）。然后将胚胎孵化至出壳。方法A、B、C、D和E培养的胚胎的孵化率分别为34.4%、16.2%、50.0%、6.9 - 26.7%和47.5%。因此，在胚盘阶段用稀蛋白替代浓稠蛋白可提高培养受精卵的孵化率。通过方法A获得的小鸡发育成熟并产生了可存活的后代，并且该技术为受精卵培养至孵化提供了一种改进方法。
