Dunn B E, Boone M A
Poult Sci. 1976 May;55(3):1067-71. doi: 10.3382/ps.0551067.
An improved technique is described for in vitro culture of the chick embryo from three days through 21 days of total incubation (three days in shell plus 18 days in culture). Mean survival time for cultured embryos is 18 days of total incubation, and mean morphological stage is Hamburger-Hamilton stage 41 (15 days). Mean measurements of wet weight, dry weight, and right third toe length for stage 45 (partial or complete abdominal yolk sac retraction, 19-20 days) cultured embryos are comparable with corresponding means for stage 42-43 (16-17 days) embryos from control intact eggs. Possible causes of growth retardation in vitro are briefly discussed.
本文描述了一种改进的技术，用于从孵化三天到整个孵化期21天（壳内三天加培养18天）的鸡胚体外培养。培养胚胎的平均存活时间为整个孵化期的18天，平均形态学阶段为汉密尔顿-汉伯格第41阶段（15天）。第45阶段（部分或完全腹侧卵黄囊回缩，19 - 20天）培养胚胎的湿重、干重和右第三趾长度的平均测量值与对照完整鸡蛋中第42 - 43阶段（16 - 17天）胚胎的相应平均值相当。简要讨论了体外生长迟缓的可能原因。
