Mao Qiang, Shao Weijie, Lv Shuaijie, Tong Peijian, He Bangjian
Department of Orthopedics, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
The First School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, China.
Front Med (Lausanne). 2022 Aug 18;9:945268. doi: 10.3389/fmed.2022.945268. eCollection 2022.
This study aimed to explore whether peripheral blood stem cells (PBSCs) infused through the medial circumflex femoral artery to treat osteonecrosis of the femoral head (ONFH) could migrate into the necrotic area of femoral head.
We collected PBSCs from a patient who had bilateral ONFH by apheresis technique using COBE spectra apheresis system (COBE BCT Inc, Lakewood, CO, USA) after subcutaneous injections of granulocyte-colony stimulating factor (G-CSF) at a dosage of 10 μg/kg for 4 days to mobilize PBSCs. After that, 100 MBq 2-[F]-fluoro-2-deoxy-D-glucose (F-FDG) was used to label PBSCs. F-FDG labeled PBSCs were infused into the left femoral head the medial circumflex femoral artery to treat ONFH. Then the patient was underwent three-dimensional positron emission tomography (3D-PET) examination 60 min after cell infusion to monitor the biological distribution of F-FDG-labeled PBSCs, and to observe whether the transplanted PBSCs could migrate into the necrotic area of femoral head.
The total number of monouclear cells in the peripheral blood stem cell suspension was 1.95 × 10 which contained 2.20 × 10 CD34 cells. The activity of F-FDG in the labeled cells was 1.8Bq/10 monouclear cells. 3D-PET imaging showed that F-FDG radioactivity was detected in the necrotic area of femoral head, acetabulum and femoral bone marrow cavity after transplantation of F-FDG-labeled PBSCs via the medial circumflex femoral artery. It is worth noting that although PBSCs labeled with F-FDG were widely distributed around the hip, such as femoral bone marrow cavity, femoral head and acetabulum, PBSCs were generally located in the necrotic area of femoral head.
PBSCs could enter into the femoral head and migrate into the necrotic field of femoral head participating in the repair of osteonecrosis after infusion through the medial circumflex femoral artery.
本研究旨在探讨经旋股内侧动脉输注外周血干细胞（PBSCs）治疗股骨头坏死（ONFH）时，这些细胞是否能迁移至股骨头坏死区域。
我们从一名双侧股骨头坏死患者身上采集PBSCs。在皮下注射剂量为10μg/kg的粒细胞集落刺激因子（G-CSF）4天以动员PBSCs后，使用COBE spectra血细胞分离系统（美国科罗拉多州莱克伍德市COBE BCT公司）通过血细胞分离技术采集。之后，用100MBq的2-[F]-氟-2-脱氧-D-葡萄糖（F-FDG）标记PBSCs。将F-FDG标记的PBSCs经旋股内侧动脉注入左侧股骨头以治疗ONFH。细胞输注60分钟后，对患者进行三维正电子发射断层扫描（3D-PET）检查，以监测F-FDG标记的PBSCs的生物分布，并观察移植的PBSCs是否能迁移至股骨头坏死区域。
外周血干细胞悬液中单个核细胞总数为1.95×10，其中包含2.20×10个CD34细胞。标记细胞中F-FDG的活性为1.8Bq/10个单个核细胞。3D-PET成像显示，经旋股内侧动脉移植F-FDG标记的PBSCs后，可以在股骨头坏死区域、髋臼和股骨髓腔内检测到F-FDG放射性。值得注意的是，尽管用F-FDG标记的PBSCs广泛分布于髋关节周围，如股骨髓腔、股骨头和髋臼，但PBSCs通常位于股骨头坏死区域。
PBSCs经旋股内侧动脉输注后可进入股骨头并迁移至股骨头坏死区域，参与骨坏死的修复。
