Nagar Padamnabhi S, Rane Shailendra, Dwivedi Mannu
Department of Botany, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India.
Shimadzu Analytical (India) Pvt. Ltd., 1 A/B Rushabh Chambers, Makwana Road, Marol, Andheri (E), Mumbai 400059, Maharashtra, India.
Heliyon. 2022 Aug 15;8(8):e10234. doi: 10.1016/j.heliyon.2022.e10234. eCollection 2022 Aug.
Glycyrrhizin is a triterpene glycoside derived from and related species which is a renowned phytochemical used to cure a variety of ailments such as inflammation, sore throat, hepatitis etc. It is in huge demand owing to its various valuable properties. With the ever-increasing demand of glycyrrhizin, the search for alternative sources for glycyrrhizin is on rise. One such species with a scientific basis and good concentration of glycyrrhizin is A thin-layer chromatography (TLC) method was established to determine the presence of glycyrrhizin in . Further, standardisation and validation, a High performance liquid chromatography (model NEXERA-X2) with LCMS system (Model LCMS-8040) from Shimadzu were used. The analysis was performed by using shim-pack XR-ODS, C18 (75 mm × 3.0 mm) 2.2 μm. In this analysis, the mobile phase used was a combination of acetonitrile and a 20 mM ammonium acetate buffer that was subjected to gradient time programming and monitored by Multiple Reaction Monitoring (MRM) in positive ion mode. The method was validated for linearity, accuracy, precision, recovery, detection, and quantitation limit. The technique was confirmed to be linear within the concentration range of 5 ng/mL to 500 ng/mL with R > 0.991. The LOD and the LOQ were 2 ng/mL and 5 ng/mL respectively. The suggested approach satisfied the acceptance criteria for linearity, accuracy, precision, specificity, robustness, LOD, LOQ, and system adaptability.
甘草甜素是一种从甘草及相关物种中提取的三萜糖苷，是一种著名的植物化学物质，用于治疗多种疾病，如炎症、喉咙痛、肝炎等。由于其多种宝贵特性，对它的需求量极大。随着对甘草甜素需求的不断增加，寻找甘草甜素的替代来源的研究也在增加。一种具有科学依据且甘草甜素含量较高的物种是[具体物种未给出]。建立了一种薄层色谱（TLC）方法来测定[具体物种未给出]中甘草甜素的存在。此外，进行了标准化和验证，使用了岛津公司的带有LCMS系统（型号LCMS - 8040）的高效液相色谱仪（型号NEXERA - X2）。分析使用的是shim - pack XR - ODS，C18（75mm×3.0mm）2.2μm色谱柱。在该分析中，流动相是乙腈和20mM醋酸铵缓冲液的组合，进行梯度时间程序设置，并在正离子模式下通过多反应监测（MRM）进行监测。该方法针对线性、准确性、精密度、回收率、检测限和定量限进行了验证。该技术在5ng/mL至500ng/mL的浓度范围内被确认为线性，R＞0.991。检测限和定量限分别为2ng/mL和5ng/mL。所建议的方法满足了线性、准确性、精密度、特异性、稳健性、检测限、定量限和系统适应性的验收标准。
