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用于检测和生态监测水稻重要种传病原菌的比色环介导等温扩增检测法

Colorimetric loop-mediated isothermal amplification assay for detection and ecological monitoring of , an important seed-borne pathogen of rice.

作者信息

Choudhary Prassan, Goswami Sanjay Kumar, Chakdar Hillol, Verma Shaloo, Thapa Shobit, Srivastava Alok Kumar, Saxena Anil Kumar

机构信息

Microbial Technology Unit II, ICAR-National Bureau of Agriculturally Important Microorganisms, Mau, India.

Indian Council of Agricultural Research (ICAR)-Indian Institute of Sugarcane Research, Lucknow, India.

出版信息

Front Plant Sci. 2022 Aug 18;13:936766. doi: 10.3389/fpls.2022.936766. eCollection 2022.

DOI:10.3389/fpls.2022.936766
PMID:36061774
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9434274/
Abstract

Accurate and timely disease detection plays a critical role in achieving sustainable crop protection. Globally, rice has been a staple crop for centuries plagued by the diseases that greatly hamper its productivity. Sheath rot, an emerging disease of rice caused by the seed-borne pathogen , has reportedly caused heavy losses to agricultural produce in recent years. Our study has led to the development and validation of a LAMP assay for early detection of , the causal agent of sheath rot from the live-infected tissues, seeds, weeds, and environmental samples. The assay could detect as low as 1.6 fg/μl of the pathogen in 15 min. The assay was implemented to bio-surveil the presence of this pathogen by testing it on three weed species (, , and ) growing around the rice fields. The results showed the presence of the pathogen in two of the weed species . and . The assay was used to test 13 different rice varieties for the presence of in seeds. In total, three of the varieties did not show the presence of in their seeds while the rest were found to harbor the pathogen. The developed assay can effectively be used to detect and screen the presence of in live samples including seeds and field soil.

摘要

准确及时的病害检测在实现可持续作物保护方面发挥着关键作用。在全球范围内，几个世纪以来，水稻一直是主要作物，但却饱受严重影响其生产力的病害困扰。鞘腐病是一种由种子传播病原体引起的水稻新出现病害，据报道近年来已给农产品造成了重大损失。我们的研究促成了一种环介导等温扩增（LAMP）检测方法的开发与验证，用于从活体感染组织、种子、杂草和环境样本中早期检测鞘腐病的病原体。该检测方法在15分钟内可检测低至1.6 fg/μl的病原体。通过在稻田周围生长的三种杂草物种（[杂草物种1]、[杂草物种2]和[杂草物种3]）上进行测试，实施该检测方法对这种病原体的存在进行生物监测。结果表明在两种杂草物种[杂草物种1]和[杂草物种2]中存在该病原体。该检测方法用于测试13个不同水稻品种种子中是否存在[病原体名称]。总共，其中三个品种的种子未检测到[病原体名称]，而其余品种被发现携带该病原体。所开发的检测方法可有效地用于检测和筛选包括种子和田间土壤在内的活体样本中[病原体名称]的存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/3ef5f95c882b/fpls-13-936766-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/6bc3c7d61316/fpls-13-936766-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/791e6d89859a/fpls-13-936766-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/4fd183b9a9cb/fpls-13-936766-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/67137045099c/fpls-13-936766-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/3ef5f95c882b/fpls-13-936766-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/6bc3c7d61316/fpls-13-936766-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/791e6d89859a/fpls-13-936766-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/4fd183b9a9cb/fpls-13-936766-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/67137045099c/fpls-13-936766-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c39d/9434274/3ef5f95c882b/fpls-13-936766-g005.jpg

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Identification of Rice Seed-Derived Spp. and Development of LAMP Assay against .
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