甘油通过调节植物脂肪酸代谢、植物激素交叉对话和病程相关基因诱导小麦抗白粉病。
Glycerol-Induced Powdery Mildew Resistance in Wheat by Regulating Plant Fatty Acid Metabolism, Plant Hormones Cross-Talk, and Pathogenesis-Related Genes.
机构信息
Key Laboratory of Crop Heterosis and Utilization (MOE) and State Key Laboratory for Agrobiotechnology, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China.
Institute of Evolution, University of Haifa, 199 Abba-Hushi Avenue, Mt. Carmel, Haifa 3498838, Israel.
出版信息
Int J Mol Sci. 2020 Jan 20;21(2):673. doi: 10.3390/ijms21020673.
Our previous study indicated that glycerol application induced resistance to powdery mildew () in wheat by regulating two important signal molecules, glycerol-3-phosphate (G3P) and oleic acid (OA18:1). Transcriptome analysis of wheat leaves treated by glycerol and inoculated with was performed to identify the activated immune response pathways. We identified a set of differentially expressed transcripts (e.g., , , and ) involved in glycerol and fatty acid metabolism that were upregulated in response to infection and might contribute to G3P and OA18:1 accumulation. Gene Ontology (GO) enrichment analysis revealed GO terms induced by glycerol, such as response to jasmonic acid (JA), defense response to bacterium, lipid oxidation, and growth. In addition, glycerol application induced genes (e.g., , , and ) involved in the metabolism pathway of linolenic and alpha-linolenic acid, which are precursor molecules of JA biosynthesis. Glycerol induced JA and salicylic acid (SA) levels, while glycerol reduced the auxin (IAA) level in wheat. Glycerol treatment also induced pathogenesis related () genes, including , , , , , , , , , etc. These results indicate that glycerol treatment regulates fatty acid metabolism and hormones cross-talk and induces the expression of genes that together contribute to resistance in wheat.
我们之前的研究表明，通过调节两种重要的信号分子甘油-3-磷酸（G3P）和油酸（OA18:1），甘油的应用诱导小麦对白粉病（）产生抗性。对用甘油处理并用接种的小麦叶片进行转录组分析，以鉴定被激活的免疫反应途径。我们鉴定了一组差异表达的转录本（例如、、和），它们参与甘油和脂肪酸代谢，对感染的反应上调，可能有助于 G3P 和 OA18:1 的积累。基因本体论（GO）富集分析揭示了甘油诱导的 GO 术语，如对茉莉酸（JA）的反应、对细菌的防御反应、脂质氧化和生长。此外，甘油应用诱导参与亚麻酸和α-亚麻酸代谢途径的基因（例如、、和），它们是茉莉酸生物合成的前体分子。甘油诱导 JA 和水杨酸（SA）水平，而甘油降低小麦中的生长素（IAA）水平。甘油处理还诱导病程相关（）基因的表达，包括、、、、、、、、等。这些结果表明，甘油处理调节脂肪酸代谢和激素串扰，并诱导参与小麦抗性的基因的表达。
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