保护零价铁纳米颗粒免受脓毒症和脓毒性心力衰竭的影响。
Protection of zero-valent iron nanoparticles against sepsis and septic heart failure.
机构信息
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences and Medicine, Northwest University, 229 Taibai North Road, Xi'an, China.
School of Chemistry, Xi'an Jiaotong University, No. 28, West Xianning Road, 710049, Xi'an, Shaanxi, China.
出版信息
J Nanobiotechnology. 2022 Sep 5;20(1):405. doi: 10.1186/s12951-022-01589-1.
BACKGROUND
Septic heart failure accounts for high mortality rates globally. With a strong reducing capacity, zero-valent iron nanoparticles (nanoFe) have been applied in many fields. However, the precise roles and mechanisms of nanoFe in septic cardiomyopathy remain unknown.
RESULTS
NanoFe was prepared via the liquid-phase reduction method and functionalized with the biocompatible polymer sodium carboxymethylcellulose (CMC). We then successfully constructed a mouse model of septic myocardial injury by challenging with cecal ligation and puncture (CLP). Our findings demonstrated that nanoFe has a significant protective effect on CLP-induced septic myocardial injury. This may be achieved by attenuating inflammation and oxidative stress, improving mitochondrial function, regulating endoplasmic reticulum stress, and activating the AMPK pathway. The RNA-seq results supported the role of nanoFe treatment in regulating a transcriptional profile consistent with its role in response to sepsis.
CONCLUSIONS
The results provide a theoretical basis for the application strategy and combination of nanoFe in sepsis and septic myocardial injury.
背景
败血性心力衰竭在全球范围内的死亡率很高。零价铁纳米粒子（nanoFe）具有很强的还原能力，已被应用于许多领域。然而，nanoFe 在败血性心肌病中的确切作用和机制尚不清楚。
结果
通过液相还原法制备纳米 Fe，并通过生物相容性聚合物羧甲基纤维素钠（CMC）对其进行功能化。然后，我们通过盲肠结扎和穿刺（CLP）成功构建了小鼠败血性心肌损伤模型。我们的研究结果表明，纳米 Fe 对 CLP 诱导的败血性心肌损伤具有显著的保护作用。这可能是通过减轻炎症和氧化应激、改善线粒体功能、调节内质网应激以及激活 AMPK 通路来实现的。RNA-seq 结果支持纳米 Fe 处理在调节与应对败血症一致的转录谱方面的作用。
结论
这些结果为纳米 Fe 在败血症和败血性心肌损伤中的应用策略和联合治疗提供了理论依据。
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