Department of Physiology and Pharmacology, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, T2N 4N1, Canada.
International Microbiome Centre, Cumming School of Medicine, University of Calgary, Calgary, AB, T2N 4N1, Canada.
Mucosal Immunol. 2022 Jun;15(6):1085-1094. doi: 10.1038/s41385-022-00562-3. Epub 2022 Sep 5.
The gut microbiota influences host responses at practically every level, and as research into host-microbe interactions expands, it is not surprising that we are uncovering similar roles for the microbiota at other barrier sites, such as the lung and skin. Using standard laboratory mice to assess host-microbe interactions, or even host intrinsic responses, can be challenging, as slight variations in the microbiota can affect experimental outcomes. When it comes to designing and selecting an appropriate level of microbial diversity and community structure for colonization of our laboratory rodents, we have more choices available to us than ever before. Here we will discuss the different approaches used to modulate microbial complexity that are available to study host-microbe interactions. We will describe how different models have been used to answer distinct biological questions, covering the entire microbial spectrum, from germ-free to wild.
肠道微生物群在几乎所有层面上影响宿主反应，随着对宿主-微生物相互作用的研究不断扩展，我们在其他屏障部位（如肺部和皮肤）发现微生物群具有相似作用也就不足为奇了。使用标准实验室小鼠来评估宿主-微生物相互作用，甚至是宿主内在反应，可能具有挑战性，因为微生物群的微小变化会影响实验结果。在设计和选择适合我们实验室啮齿动物定植的微生物多样性和群落结构水平时，我们拥有比以往更多的选择。在这里，我们将讨论可用于研究宿主-微生物相互作用的调节微生物复杂性的不同方法。我们将描述不同模型如何用于回答不同的生物学问题，涵盖了从无菌到野生的整个微生物范围。
