Wang Jingge, Zheng Zibin, Yang Hua, Chen Jie, Xiao Yingping, Ji Xiaofeng, Zhang Zhenming, He Hailian, Ding Baoan, Tang Biao
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products & Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China.
AMB Express. 2022 Sep 6;12(1):115. doi: 10.1186/s13568-022-01458-y.
β-1,3/1,6-glucan as a prebiotic improves immune performance in animals. These functions are closely related to the effect of β-1,3/1,6-glucan on gut microbiota structure. However, the effect of β-1,3/1,6-glucan on the gut microbiota structure of broilers is unclear. The aim of this study was to confirm the effects of β-1,3/1,6-glucan on the cecal microflora structure of yellow-feathered broilers. This study monitored the antimicrobial resistance (AMR) level of Escherichia coli in feces of yellow-feathered broilers by standard broth dilution method and mastered the AMR level of chickens selected. The effects of β-1,3/1,6-glucan on gut microbiota were investigated by 16S rRNA sequencing. The results showed that the number of isolated multidrug-resistant E. coli strains accounted for 98.41%. At 14, 21, and 28 days of age, supplemented of 0.2%, 0.1%, and 0.1% β-1,3/1,6-glucan in yellow-feathered broiler diets significantly altered gut microbial composition, and beneficial bacteria Alistipes, Bacteroides and Faecalibacterium were significantly increased. These findings provide guidance and recommendations for β-1,3/1,6-glucan as a broiler feed additive to improve the growth of broilers.
β-1,3/1,6-葡聚糖作为一种益生元可改善动物的免疫性能。这些功能与β-1,3/1,6-葡聚糖对肠道微生物群结构的影响密切相关。然而，β-1,3/1,6-葡聚糖对肉鸡肠道微生物群结构的影响尚不清楚。本研究的目的是确认β-1,3/1,6-葡聚糖对黄羽肉鸡盲肠微生物区系结构的影响。本研究采用标准肉汤稀释法监测黄羽肉鸡粪便中大肠杆菌的抗菌耐药性（AMR）水平，并掌握所选鸡的AMR水平。通过16S rRNA测序研究β-1,3/1,6-葡聚糖对肠道微生物群的影响。结果表明，分离出的多重耐药大肠杆菌菌株数量占98.41%。在14、21和28日龄时，在黄羽肉鸡日粮中添加0.2%、0.1%和0.1%的β-1,3/1,6-葡聚糖可显著改变肠道微生物组成，有益菌阿里斯杆菌、拟杆菌和粪杆菌显著增加。这些发现为β-1,3/1,6-葡聚糖作为肉鸡饲料添加剂促进肉鸡生长提供了指导和建议。
