Bochani Shayesteh, Kalantari-Hesari Ali, Haghi Fakhri, Alinezhad Vajihe, Bagheri Hadi, Makvandi Pooyan, Shahbazi Mohammad-Ali, Salimi Abdollah, Hirata Ikue, Mattoli Virgilio, Maleki Aziz, Guo Baolin
Zanjan Pharmaceutical Nanotechnology Research Center (ZPNRC), Zanjan 45139-56184, Iran.
Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan 45139-56184, Iran.
ACS Appl Bio Mater. 2022 Sep 6. doi: 10.1021/acsabm.2c00567.
The design and development of multifunctional injectable hydrogels with high photothermal antibacterial activity and shape adaptability to accelerate bacteria-infected wound healing is of critical importance in clinical applications. In this study, a hybrid hydrogel composed of gelatin, iron, and MnO nanosheets was prepared by multiple interactions, including coordinative and hydrogen bonding as well as electrostatic attraction. The introduced MnO and Fe components made the hydrogels photothermally and chemodynamically active, thereby endowing them with potent antibacterial capabilities against both Gram-negative and Gram-positive bacteria. Because of the Fenton activity of the hydrogels, they could produce abandoned oxygen, which is highly crucial in the healing process of wounds. They also showed good cytocompatibility and hemocompatibility as well as high hemostatic properties. Moreover, the injectable hydrogels could fill irregular wounds and significantly accelerate bacteria-infected wound healing through decreasing the inflammatory response and increasing blood vessels. These features indicated the promising potential of the multifunctional hydrogel for healing infected full-thickness wounds.
设计和开发具有高光热抗菌活性和形状适应性以加速细菌感染伤口愈合的多功能可注射水凝胶在临床应用中至关重要。在本研究中，通过配位、氢键以及静电吸引等多种相互作用制备了一种由明胶、铁和MnO纳米片组成的混合水凝胶。引入的MnO和Fe成分使水凝胶具有光热和化学动力学活性，从而赋予它们对革兰氏阴性菌和革兰氏阳性菌强大的抗菌能力。由于水凝胶的芬顿活性，它们可以产生超氧阴离子，这在伤口愈合过程中至关重要。它们还表现出良好的细胞相容性和血液相容性以及高止血性能。此外，可注射水凝胶可以填充不规则伤口，并通过减少炎症反应和增加血管生成来显著加速细菌感染伤口的愈合。这些特性表明多功能水凝胶在治疗感染的全层伤口方面具有广阔的应用前景。
