CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China; Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, 264003, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Talanta. 2024 Feb 1;268(Pt 1):125293. doi: 10.1016/j.talanta.2023.125293. Epub 2023 Oct 12.
Hydrogen polysulfide (HS, n > 1) is an important component of reactive sulfur species (RSS), which is an important substance for maintaining the redox balance in cells. However, limited recognition moieties are available for hydrogen polysulfide probe design. In this study, we have constructed a small library containing several organic molecules to explore a new specific recognition moiety for HS fluorescent probe design. To validate the discovery, two fluorescent probes, 7 and BCC, were further developed based on coumarin and its derivative. The probes exhibited desirable specificity for HS monitoring, which can be used for detecting HS in solution and cells. The new specific recognition moiety for HS fluorescent probe design discovered in this work has certain guiding significance for development of HS probes exploring biological roles in the future.
氢多硫化物（HS，n>1）是活性硫物种（RSS）的重要组成部分，是维持细胞氧化还原平衡的重要物质。然而，用于氢多硫化物探针设计的识别基团有限。在这项研究中，我们构建了一个包含几个有机分子的小分子库，以探索用于 HS 荧光探针设计的新的特异性识别基团。为了验证这一发现，我们进一步基于香豆素及其衍生物开发了两种荧光探针 7 和 BCC。这些探针对 HS 的监测表现出了理想的特异性，可用于检测溶液和细胞中的 HS。本工作中发现的用于 HS 荧光探针设计的新的特异性识别基团对未来探索 HS 在生物学中作用的 HS 探针的开发具有一定的指导意义。
