体内氟烷在脑中分布的19F-核磁共振研究。

In vivo 19F-NMR study of halothane distribution in brain.

作者信息

Wyrwicz A M, Conboy C B, Nichols B G, Ryback K R, Eisele P

出版信息

Biochim Biophys Acta. 1987 Jul 29;929(3):271-7. doi: 10.1016/0167-4889(87)90253-9.

Abstract

Halothane distribution and elimination from rabbit brain was studied in vivo using 19F-NMR spectroscopy. Two exponential decay functions for the anesthetic were observed in the clearance curve. They are assigned to halothane in brain held in two distinct chemical environments characterized by different chemical shifts, and half-lives (25 and 320 min). A nonvolatile halothane metabolite with a half-life of several days was found to be present in rabbit brains. The in vivo results were corroborated by ex vivo experiments on excised brain tissue. Halothane was distributed in all of the major cell subfractions, whereas the metabolite was present predominantly in the cytoplasm.

摘要

利用19F-核磁共振波谱技术在体内研究了氟烷在兔脑中的分布和消除情况。在清除曲线中观察到该麻醉剂有两个指数衰减函数。它们分别对应处于两种不同化学环境（以不同化学位移和半衰期（25分钟和320分钟）为特征）的脑中氟烷。发现兔脑中存在一种半衰期为数天的非挥发性氟烷代谢物。体内实验结果得到了对切除脑组织的体外实验的证实。氟烷分布于所有主要细胞亚组分中，而代谢物主要存在于细胞质中。