Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Center of excellence in Bionanoscience Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Institute of Intelligence Informatics Technology, Sangmyung University, Seoul 03016, Republic of Korea.
Gene. 2022 Dec 20;846:146853. doi: 10.1016/j.gene.2022.146853. Epub 2022 Sep 5.
Members of the genus Brevibacillus belonging to the familyPaenibacillaceae are Gram-positive/variable, endospore-forming, and rod-shaped bacteria that dwell in various environmental habitats. Brevibacillus spp. have a wide range of enzyme activities such as degradation of various carbohydrates, plastics, and they possess resistance against heavy metals. These characteristics make them encouraging contenders for biotechnological applications.In this work, we analyzed the reference genomes of 19Brevibacillusspecies, focusing on discovering the biodegradation and heavy metal resistance capabilities of this little studied genus from genomic data. The results indicate that several strain specific traits were identified. For example Brevibacillus halotolerans s-14, and Brevibacillus laterosporus DSM 25 have more glycoside hydrolases (GHs) compared to other carbohydrate-active enzymes, and therefore might be more suitable for biodegradation of carbohydrates. In contrast, strains such as Brevibacillus antibioticus TGS2-1, with a higher number of glycosyltransfereases (GTs) may aid in the biosynthesis of complex carbohydrates. Our results also suggest some correlation between heavy metal resistance and polyurethane degradation, thus indicating that heavy metal resistance strains (e.g. Brevibacillus reuszeri J31TS6) can be a promising source of enzymes for polyurethane degradation. These strain specific features make the members of this bacterial group potential candidates for further investigations with industrial implications. This work also represents the first exhaustive study of Brevibacillus at the genome scale.
短芽孢杆菌属的成员属于芽孢杆菌科，是革兰氏阳性/可变、产芽孢、杆状细菌，栖息在各种环境栖息地。短芽孢杆菌属具有广泛的酶活性，如各种碳水化合物、塑料的降解，并且它们具有对重金属的抗性。这些特性使它们成为生物技术应用的有希望的竞争者。
在这项工作中，我们分析了 19 个短芽孢杆菌种的参考基因组，重点从基因组数据中发现这个研究较少的属的生物降解和重金属抗性能力。结果表明，鉴定了几个菌株特异性特征。例如，耐盐短芽孢杆菌 s-14 和迟缓芽孢杆菌 DSM 25 与其他碳水化合物活性酶相比，具有更多的糖苷水解酶 (GHs)，因此可能更适合碳水化合物的生物降解。相比之下，如 Brevibacillus antibioticus TGS2-1 等菌株，具有更多的糖基转移酶 (GTs)，可能有助于复杂碳水化合物的生物合成。我们的结果还表明，重金属抗性和聚氨酯降解之间存在一些相关性，因此表明重金属抗性菌株（例如 Brevibacillus reuszeri J31TS6）可以成为聚氨酯降解酶的有前途的来源。这些菌株特异性特征使该细菌群的成员成为具有工业意义的进一步研究的潜在候选者。这项工作也是短芽孢杆菌属在基因组规模上的首次详尽研究。
