Bowers R W, Tighe B J
Biomaterials. 1987 May;8(3):172-6. doi: 10.1016/0142-9612(87)90059-7.
Light, SEM and histochemical techniques were used to determine the architecture and composition of elevated deposits on a variety of commercial lenses of differing bulk and surface chemistries and wear protocols. Spoilt lenses were derived from controlled lens wear trials and additional clinical sources. Elevated deposits display a complex multilayered structure, unaffected by variations in wear protocol or bulk and surface chemistries. These deposits are predominantly composed of tear-derived lipids laid down in a well-defined fashion. The primary layer is composed of unsaturated lipids, whilst the secondary and tertiary layers are dominated by cholesterol and cholesterol esters. The composition and location of deposit components is unaffected by variations in, wear protocol, chemistry of the lens matrix, or tear composition of lens wearers. These findings suggest that unsaturated lipids are responsible for the biological interfacial conversion of hydrogel lenses, reducing their compatibility with the ocular environment.
采用光学、扫描电子显微镜和组织化学技术，以确定不同体积、表面化学性质和佩戴方案的各种商用镜片上隆起沉积物的结构和组成。损坏的镜片来自受控的镜片佩戴试验和其他临床来源。隆起的沉积物呈现出复杂的多层结构，不受佩戴方案、体积和表面化学性质变化的影响。这些沉积物主要由以明确方式沉积的泪液衍生脂质组成。主要层由不饱和脂质组成，而第二层和第三层则以胆固醇和胆固醇酯为主。沉积物成分的组成和位置不受佩戴方案、镜片基质化学性质或镜片佩戴者泪液成分变化的影响。这些发现表明，不饱和脂质是水凝胶镜片生物界面转化的原因，降低了它们与眼内环境的相容性。
