Chiba Ryohei, Ebihara Yuma, Shiiya Haruhiko, Ujiie Hideki, Fujiwara-Kuroda Aki, Kaga Kichizo, Li Liming, Wakasa Satoru, Hirano Satoshi, Kato Tatsuya
Department of Cardiovascular and Thoracic Surgery, Hokkaido University Faculty of Medicine, Sapporo, Japan.
Department of Gastroenterological Surgery II, Hokkaido University Faculty of Medicine, Sapporo, Japan.
J Thorac Dis. 2022 Aug;14(8):2943-2952. doi: 10.21037/jtd-22-244.
Palpation of tumors during thoracoscopic surgery remains difficult, and identification of deep-seated tumors may be impossible. This preclinical study investigated the usefulness of a novel indocyanine green (ICG) fluorescence spectroscopy system for tumor localization.
ICG was diluted to 5.0×10 mg/mL in fetal bovine serum (FBS) and mixed with silicone resin to prepare pseudo-tumors. Sponges of different densities and a porcine lung were placed on top of the pseudo-tumors, which were examined using a novel fluorescence spectroscopy system and a near-infrared (NIR) camera. Spectra were measured for different sponge and lung thicknesses, and the lung spectra were measured during both inflation and deflation.
The fluorescence spectroscopy system was able to identify tumors at depths ≥15 mm, while the NIR system was not. The spectroscopy system also detected tumors at greater depths when the density of the intervening material was lower. Depending on the density and thickness of the intervening material, the system could detect spectra as deep as 40 mm for sponges and 30 mm for lungs.
This new fluorescence spectroscopy system can be used to identify lung tumors up to a depth of 30 mm in experiments using pseudo-tumors and a porcine lung, which may aid in tumor identification during thoracoscopic surgery.
在胸腔镜手术中触诊肿瘤仍然很困难，可能无法识别深部肿瘤。这项临床前研究调查了一种新型吲哚菁绿（ICG）荧光光谱系统在肿瘤定位方面的实用性。
将ICG在胎牛血清（FBS）中稀释至5.0×10 mg/mL，并与硅树脂混合以制备假肿瘤。将不同密度的海绵和猪肺置于假肿瘤上方，使用新型荧光光谱系统和近红外（NIR）相机对其进行检查。测量不同海绵和肺厚度的光谱，并在充气和放气过程中测量肺光谱。
荧光光谱系统能够识别深度≥15 mm的肿瘤，而近红外系统则不能。当中间材料的密度较低时，光谱系统也能在更深的深度检测到肿瘤。根据中间材料的密度和厚度，该系统对于海绵可检测到深达40 mm的光谱，对于肺可检测到深达30 mm的光谱。
在使用假肿瘤和猪肺的实验中，这种新型荧光光谱系统可用于识别深度达30 mm的肺肿瘤，这可能有助于胸腔镜手术中的肿瘤识别。
