单个红细胞中膜运动的准弹性光散射研究。
Quasi-elastic light scattering studies of membrane motion in single red blood cells.
作者信息
Tishler R B, Carlson F D
出版信息
Biophys J. 1987 Jun;51(6):993-7. doi: 10.1016/S0006-3495(87)83428-8.
Abstract
Studies of red blood cells (RBCs) and RBC ghosts, using a quasi-elastic light scattering (QELS) microscope spectrometer, have identified the membrane as the primary source of the light scattering signal. This is the first report in which motion of the cell membrane has been demonstrated to be the primary source of the QELS signal from a cell. Cytoplasmic changes induced in the RBC by varying the osmotic strength of the medium were also detected using this technique. Comparison of the data from white blood cells (WBCs) with the RBC data demonstrated significant differences between different types of cells.
摘要
利用准弹性光散射（QELS）显微镜光谱仪对红细胞（RBC）和红细胞血影进行的研究已经确定细胞膜是光散射信号的主要来源。这是首次有报告证明细胞膜的运动是细胞QELS信号的主要来源。使用该技术还检测了通过改变培养基渗透强度在红细胞中诱导产生的细胞质变化。将白细胞（WBC）的数据与红细胞的数据进行比较，结果表明不同类型的细胞之间存在显著差异。
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本文引用的文献
1
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2
Kinetics of sickle haemoglobin polymerization in single red cells.单个红细胞中镰状血红蛋白聚合的动力学
Nature. 1982 Nov 11;300(5888):194-7. doi: 10.1038/300194a0.
3
Influence of physicochemical factors on rheology of human neutrophils.物理化学因素对人中性粒细胞流变学的影响。
Biophys J. 1982 Jul;39(1):101-6. doi: 10.1016/S0006-3495(82)84495-0.
4
Quasi-elastic light-scattering studies of single skeletal muscle fibers.单根骨骼肌纤维的准弹性光散射研究。
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5
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6
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7
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