Ali Basharat, Saleem Muhammad Hamzah, Ali Shafaqat, Shahid Munazzam, Sagir Muhammad, Tahir Muhammad Bilal, Qureshi Kamal Ahmad, Jaremko Mariusz, Selim Samy, Hussain Afzal, Rizwan Muhammad, Ishaq Wajid, Rehman M Zia-Ur
Khwaja Fareed University of Engineering and Information Technology (KFUEIT), Rahim Yar Khan, Pakistan.
Faculty of Agriculture, University of Agriculture, Faisalabad, Pakistan.
Front Plant Sci. 2022 Aug 22;13:973782. doi: 10.3389/fpls.2022.973782. eCollection 2022.
Salinity has become a major environmental concern of agricultural lands, impairing crop production. The current study aimed to examine the role of zinc oxide nanoparticles (ZnO NPs) in reducing the oxidative stress induced by salinity and the overall improvement in phytochemical properties in barley. A total of nine different barley genotypes were first subjected to salt (NaCl) stress in hydroponic conditions to determine the tolerance among the genotypes. The genotype Annora was found as most sensitive, and the most tolerant genotype was Awaran 02 under salinity stress. In another study, the most sensitive (Annora) and tolerant (Awaran 02) barley genotypes were grown in pots under salinity stress (100 mM). At the same time, half of the pots were provided with the soil application of ZnO NPs (100 mg kg), and the other half pots were foliar sprayed with ZnO NPs (100 mg L). Salinity stress reduced barley growth in both genotypes compared to control plants. However, greater reduction in barley growth was found in Annora (sensitive genotype) than in Awaran 02 (tolerant genotype). The exogenous application of ZnO NPs ameliorated salt stress and improved barley biomass, photosynthesis, and antioxidant enzyme activities by reducing oxidative damage caused by salt stress. However, this positive effect by ZnO NPs was observed more in Awaran 02 than in Annora genotype. Furthermore, the foliar application of ZnO NPs was more effective than the soil application of ZnO NPs. Findings of the present study revealed that exogenous application of ZnO NPs could be a promising approach to alleviate salt stress in barley genotypes with different levels of salinity tolerance.
盐度已成为农田主要的环境问题，影响作物产量。本研究旨在探讨氧化锌纳米颗粒（ZnO NPs）在减轻盐度诱导的氧化应激以及全面改善大麦植物化学特性方面的作用。首先，在水培条件下对9种不同的大麦基因型进行盐（NaCl）胁迫处理，以确定各基因型之间的耐受性。结果发现，基因型Annora最为敏感，而在盐胁迫下最耐受的基因型是Awaran 02。在另一项研究中，将最敏感（Annora）和最耐受（Awaran 02）的大麦基因型种植在盐胁迫（100 mM）的花盆中。同时，一半花盆通过土壤施用ZnO NPs（100 mg/kg），另一半花盆进行叶面喷施ZnO NPs（100 mg/L）。与对照植株相比，盐胁迫降低了两种基因型大麦的生长。然而，Annora（敏感基因型）的大麦生长减少幅度大于Awaran 02（耐受基因型）。ZnO NPs的外源施用通过减少盐胁迫引起的氧化损伤，减轻了盐胁迫并改善了大麦生物量、光合作用和抗氧化酶活性。然而，ZnO NPs的这种积极作用在Awaran 02中比在Annora基因型中更为明显。此外，叶面喷施ZnO NPs比土壤施用ZnO NPs更有效。本研究结果表明，外源施用ZnO NPs可能是缓解不同耐盐水平大麦基因型盐胁迫的一种有前景的方法。
