College of Veterinary, South China Agricultural University, Guangzhou 510642, China; College of Materials and energy, South China Agricultural University, Guangzhou 510642, China.
College of Materials and energy, South China Agricultural University, Guangzhou 510642, China.
Int J Biol Macromol. 2022 Nov 30;221:1259-1270. doi: 10.1016/j.ijbiomac.2022.09.013. Epub 2022 Sep 6.
The treatment of traumatic spinal cord injury (SCI) remains challenging as the neuron regeneration is impaired by irregular cavity and apoptosis. An injectable in situ gelling hydrogel is therefore developed for the local delivery of cannabidiol (CBD) through a novel method based on polyelectrolyte (PEC) interaction of sodium carboxymethylcellulose (CMC) and chitosan (CS). It can be injected into the spinal cord cavity with a 26-gauge syringe before gelation, and gelled after 110 ± 10 s. Of note, the in-situ forming hydrogel has mechanical properties similar to spinal cord. Moreover, the CBD-loaded hydrogels sustain delivery of CBD for up to 72 h, resulting in reducing apoptosis in SCI by enhancing mitochondrial biogenesis. Importantly, the CBD-loaded hydrogels raise neurogenesis more than pure hydrogels both in vivo and in vitro, further achieving significant recovery of motor and urinary function in SCI rats. Thus, it suggested that CMC/CS/CBD hydrogels could be used as promising biomaterials for tissue engineering and SCI.
创伤性脊髓损伤 (SCI) 的治疗仍然具有挑战性，因为神经元再生受到不规则腔和细胞凋亡的损害。因此，通过基于聚电解质 (PEC) 相互作用的新型方法，开发了一种可注射的原位凝胶水凝胶，用于通过局部递送大麻二酚 (CBD)。它可以在凝胶形成之前用 26 号注射器注入脊髓腔，并在 110±10 秒后凝胶化。值得注意的是，原位形成的水凝胶具有类似于脊髓的机械性能。此外，负载 CBD 的水凝胶可持续释放 CBD 长达 72 小时，通过增强线粒体生物发生来减少 SCI 中的细胞凋亡。重要的是，负载 CBD 的水凝胶在体内和体外均比纯水凝胶更能促进神经发生，从而使 SCI 大鼠的运动和排尿功能得到显著恢复。因此，建议 CMC/CS/CBD 水凝胶可用作组织工程和 SCI 的有前途的生物材料。
