University of Agricultural Sciences, Karnataka, Dharwad, 580 005, India.
Department of Nanotechnology, University of Agricultural Science, Dharwad, India.
Biometals. 2022 Dec;35(6):1255-1269. doi: 10.1007/s10534-022-00440-2. Epub 2022 Sep 8.
In order to achieve the food demand of a growing population, agricultural productivity needs to be increased by employing safe strategies. In the present study we have evaluated ZnONPs that were synthesized from the culture supernatant of Bacillus subtilis. Bio mimetically synthesized ZnONPs showed a surface resonance peak of 355 nm corresponding to NPs formation. Further, NPs were examined for their size, shape and element confirmation by DLS, AFM, SEM, TEM and EDAX, which confirmed the synthesized NPs were nearly spherical in size with average diameter of 32 nm by TEM. Surface charge of + 34.3 mV was observed for NPs with a low poly-dispersity index of 0.21. In vitro efficacy studies against fungi Colletotrichum capsici, Sclerotium rolfsii, Alternaria solani and Fusarium oxysporum f. sp. cicero showed up to 99% mycelial growth inhibition at 0.125% ZnONPs. Further, in-vitro disk-diffusion assay showed inhibition zones of 23 ± 0.4 mm and 12.67 ± 0.24 mm for Xanthomonas axonopodis pv. punicae (Xap) and Xanthomonas oryzae pv. oryzae (Xoo) bacterial cultures. Plant toxicity study was observed that ≤ 0.14% NPs concentration was safe under greenhouse conditions. Overall, the present study emphasizes the potential effect of ZnONPs against agricultural pathogens which play an important role in agriculture production.
为了满足不断增长的人口的粮食需求，需要采用安全的策略来提高农业生产力。在本研究中，我们评估了由枯草芽孢杆菌培养液合成的 ZnONPs。生物模拟合成的 ZnONPs 显示出 355nm 的表面共振峰，对应于 NPs 的形成。此外，通过 DLS、AFM、SEM、TEM 和 EDAX 对 NPs 的尺寸、形状和元素进行了检查，这些检查证实合成的 NPs 几乎呈球形，TEM 平均直径为 32nm。NPs 的表面电荷为+34.3mV，多分散指数为 0.21。体外对真菌辣椒炭疽菌、核盘菌、茄丝核盘菌和尖孢镰刀菌的功效研究表明，在 0.125%ZnONPs 下，菌丝生长抑制率高达 99%。此外，体外圆盘扩散试验显示，对番石榴黄单胞菌（Xap）和水稻白叶枯病菌（Xoo）的抑菌圈分别为 23±0.4mm 和 12.67±0.24mm。在温室条件下观察到植物毒性研究，≤0.14%的 NPs 浓度是安全的。总的来说，本研究强调了 ZnONPs 对农业病原菌的潜在影响，这些病原菌在农业生产中起着重要作用。
