Mannino Giuseppe, Serio Graziella, Gaglio Raimondo, Busetta Gabriele, La Rosa Lorenza, Lauria Antonino, Settanni Luca, Gentile Carla
Innovation Centre, Department of Life Sciences and Systems Biology, University of Turin, Via Quarello 15/A, 10135 Turin, Italy.
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, 90128 Palermo, Italy.
Foods. 2022 Aug 27;11(17):2605. doi: 10.3390/foods11172605.
In the context of the contemporary research on sustainable development and circular economy, the quest for effective strategies aimed at revaluation of waste and by-products generated in industrial and agricultural production becomes important. In this work, an ethanolic extract from red raspberry () seed waste (WRSP) was evaluated for its phytochemical composition and functional properties in term of antioxidative, antiproliferative, and antimicrobial activities. Chemical composition of the extract was determined by both HPLC-ESI-MS/MS and spectrophotometric methods. Phytochemical analysis revealed that flavan-3-ols and flavonols were the major phenolic compounds contained in WRSP. The extract demonstrated very high radical-scavenging (4.86 ± 0.06 µmol TE/DW) and antioxidant activity in a cell-based model (0.178 ± 0.03 mg DW/mL cell medium). The WRSP extract also exhibited antiproliferative activity against three different epithelial cancer cell lines (MCF-7, HepG2, and HeLa cells) in a dose-dependent manner. Finally, microbiological assays showed the absence of colonies of bacteria and microscopic fungi (yeasts and molds) and revealed that the WRSP extract has a large inhibition spectrum against spoilage and pathogenic bacteria, without inhibitory activity against pro-technological bacteria. In conclusion, the obtained results show that WRSP is a rich source of phytochemical compounds exerting interesting biological activities. For these reasons WRSP could find applications in the nutritional, nutraceutical, and pharmacological fields.
在当代可持续发展和循环经济研究的背景下，寻求旨在重新评估工农业生产中产生的废物和副产品的有效策略变得至关重要。在这项工作中，对红树莓（）种子废料（WRSP）的乙醇提取物进行了评估，以确定其植物化学成分以及在抗氧化、抗增殖和抗菌活性方面的功能特性。提取物的化学成分通过HPLC-ESI-MS/MS和分光光度法测定。植物化学分析表明，黄烷-3-醇和黄酮醇是WRSP中含有的主要酚类化合物。该提取物在基于细胞的模型中表现出非常高的自由基清除能力（4.86±0.06 μmol TE/DW）和抗氧化活性（0.178±0.03 mg DW/mL细胞培养基）。WRSP提取物还对三种不同的上皮癌细胞系（MCF-7、HepG2和HeLa细胞）表现出剂量依赖性的抗增殖活性。最后，微生物学检测显示没有细菌和微观真菌（酵母和霉菌）菌落，并表明WRSP提取物对腐败菌和病原菌具有广泛的抑制谱，而对工艺用细菌没有抑制活性。总之，所得结果表明WRSP是一种富含植物化学化合物的来源，具有有趣的生物活性。基于这些原因，WRSP可在营养、营养保健和药理学领域找到应用。
